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Literature summary for 3.4.22.69 extracted from

  • Lin, P.Y.; Chou, C.Y.; Chang, H.C.; Hsu, W.C.; Chang, G.G.
    Correlation between dissociation and catalysis of SARS-CoV main protease (2008), Arch. Biochem. Biophys., 472, 34-42.
    View publication on PubMed
Search for more literature for 3.4.22.69

Cloned(Commentary)

Cloned (Comment) Organism
wild-type enzyme and C-terminally truncated proteases, expression in Escherichia coli Severe acute respiratory syndrome-related coronavirus

Protein Variants

Protein Variants Comment Organism
additional information truncation of C-terminus from 306 to 300 has no appreciable effect on the quaternary structure, and the enzyme remains catalytically active. Further deletion of Gln299 or Arg298 drastically decreases the enzyme activity to 1–2% of wild type, and the major form is a monomeric one. The catalytic constant and specificity constant (kcat/Km) of the proteases are significantly decreased in the DELTA(299–306), DELTA(298–306), and DELTA(297–306) mutants. Wild type and DELTA(300–306) proteases exist with dimer as the major form. The major form becomes monomeric in DELTA(299–306), DELTA(298–306) and DELTA(297–306) Severe acute respiratory syndrome-related coronavirus
Q299A the quaternary structures of exists in a mixture of monomeric and dimeric forms Severe acute respiratory syndrome-related coronavirus
Q299E more than 90% loss of activity Severe acute respiratory syndrome-related coronavirus
Q299K more than 90% loss of activity Severe acute respiratory syndrome-related coronavirus
Q299N more than 90% loss of activity Severe acute respiratory syndrome-related coronavirus
R298A the quaternary structures of exists in a mixture of monomeric and dimeric forms Severe acute respiratory syndrome-related coronavirus
R298A/Q299A mutant is present almost exclusively in the monomeric form Severe acute respiratory syndrome-related coronavirus
R298K mutation has no significant effect on activity Severe acute respiratory syndrome-related coronavirus
R298L mutation destroys 85% of the enzyme activity Severe acute respiratory syndrome-related coronavirus
S123A mutation does not destroy the enzyme activity, the dimeric structure remains intact Severe acute respiratory syndrome-related coronavirus
S123C mutation does not destroy the enzyme activity, the dimeric structure remains intact Severe acute respiratory syndrome-related coronavirus
S139A mutation can destroy neither the enzyme activity nor the dimeric structure Severe acute respiratory syndrome-related coronavirus
S139A mutation does not destroy the enzyme activity, the dimeric structure remains intact Severe acute respiratory syndrome-related coronavirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(303-306) Severe acute respiratory syndrome-related coronavirus
0.005
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme Q200E Severe acute respiratory syndrome-related coronavirus
0.005
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme Q299N Severe acute respiratory syndrome-related coronavirus
0.006
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(304-306) Severe acute respiratory syndrome-related coronavirus
0.01
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme R298A/Q299A Severe acute respiratory syndrome-related coronavirus
0.01
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S139A Severe acute respiratory syndrome-related coronavirus
0.011
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide wild-type enzyme Severe acute respiratory syndrome-related coronavirus
0.011
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme R298A Severe acute respiratory syndrome-related coronavirus
0.012
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S123a/R298A Severe acute respiratory syndrome-related coronavirus
0.013
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(299-306) Severe acute respiratory syndrome-related coronavirus
0.013
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme R298L Severe acute respiratory syndrome-related coronavirus
0.013
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S123A Severe acute respiratory syndrome-related coronavirus
0.013
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S123C Severe acute respiratory syndrome-related coronavirus
0.014
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(305-306) Severe acute respiratory syndrome-related coronavirus
0.015
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(300-306) Severe acute respiratory syndrome-related coronavirus
0.016
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme R298K Severe acute respiratory syndrome-related coronavirus
0.018
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme Q299A Severe acute respiratory syndrome-related coronavirus
0.021
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(297-306) Severe acute respiratory syndrome-related coronavirus
0.021
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S139A/Q299A Severe acute respiratory syndrome-related coronavirus
0.022
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(302-306) Severe acute respiratory syndrome-related coronavirus
0.03
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(298-306) Severe acute respiratory syndrome-related coronavirus
0.03
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(301-306) Severe acute respiratory syndrome-related coronavirus
0.037
-
 o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme Q299K Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type enzyme and C-terminally truncated proteases Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide + H2O
-
 Severe acute respiratory syndrome-related coronavirus 2-aminobenzoyl-TSAVLQ + SGFRK-2,4-dinitrophenyl amide
-
 ?
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide + H2O
-
 Severe acute respiratory syndrome-related coronavirus o-aminobenzoyl-TSAVLQSGFRK + 2,4-dinitroaniline
-
 ?

Subunits

Subunits Comment Organism
dimer wild type and DELTA(300–306) proteases exist with dimer as the major form. The major form becomes monomeric in DELTA(299–306), DELTA(298–306) and DELTA(297–306) Severe acute respiratory syndrome-related coronavirus
monomer wild type and DELTA(300–306) proteases exist with dimer as the major form. The major form becomes monomeric in DELTA(299–306), DELTA(298–306) and DELTA(297–306) Severe acute respiratory syndrome-related coronavirus

Synonyms

Synonyms Comment Organism
SARS-CoV main protease
-
 Severe acute respiratory syndrome-related coronavirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00004
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(297-306) Severe acute respiratory syndrome-related coronavirus
0.00021
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme R298A/Q299A Severe acute respiratory syndrome-related coronavirus
0.0006
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(298-306) Severe acute respiratory syndrome-related coronavirus
0.0007
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme Q299A Severe acute respiratory syndrome-related coronavirus
0.001
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(299-306) Severe acute respiratory syndrome-related coronavirus
0.0017
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme Q299N Severe acute respiratory syndrome-related coronavirus
0.0021
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme Q299K Severe acute respiratory syndrome-related coronavirus
0.0022
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme Q200E Severe acute respiratory syndrome-related coronavirus
0.0034
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme R298A Severe acute respiratory syndrome-related coronavirus
0.0048
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme R298L Severe acute respiratory syndrome-related coronavirus
0.0048
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S139A/Q299A Severe acute respiratory syndrome-related coronavirus
0.0069
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S123a/R298A Severe acute respiratory syndrome-related coronavirus
0.017
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(300-306) Severe acute respiratory syndrome-related coronavirus
0.017
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme R298K Severe acute respiratory syndrome-related coronavirus
0.022
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(303-306) Severe acute respiratory syndrome-related coronavirus
0.025
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(301-306) Severe acute respiratory syndrome-related coronavirus
0.027
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(304-306) Severe acute respiratory syndrome-related coronavirus
0.03
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(302-306) Severe acute respiratory syndrome-related coronavirus
0.032
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide wild-type enzyme Severe acute respiratory syndrome-related coronavirus
0.033
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S123A Severe acute respiratory syndrome-related coronavirus
0.033
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S139A Severe acute respiratory syndrome-related coronavirus
0.035
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme S123C Severe acute respiratory syndrome-related coronavirus
0.039
-
 2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide mutant enzyme DELTA(305-306) Severe acute respiratory syndrome-related coronavirus