Protein Variants | Comment | Organism |
---|---|---|
Q189A | kcat for the substrate [4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] is 1.14 fold lower than wild-type value, Km-value for the substrate [4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] is 1.3fold lower than the wild-type value | Severe acute respiratory syndrome-related coronavirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(3',4'-dihydroxyphenyl)-3-beta-D-galactosyl-4H-chromen-4-one | 0.05 mM, 30.1% inhibition | Severe acute respiratory syndrome-related coronavirus | |
2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-D-arabinosyl-4H-chromen-4-one | 0.05 mM, 49.4% inhibition | Severe acute respiratory syndrome-related coronavirus | |
2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-D-galactosyl-4H-chromen-4-one | 0.05 mM, 41.8% inhibition | Severe acute respiratory syndrome-related coronavirus | |
2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-D-glucosyl-4H-chromen-4-one | 0.05 mM, 57.5% inhibition | Severe acute respiratory syndrome-related coronavirus | |
2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-L-fucosyl-4H-chromen-4-one | 0.05 mM, 57.4% inhibition | Severe acute respiratory syndrome-related coronavirus | |
2-(3',4'-dihydroxyphenyl)-5-hydroxy-3,7-di(beta-D-galactosyl)-4H-chromen-4-one | 0.05 mM, 53.0% inhibition | Severe acute respiratory syndrome-related coronavirus | |
2-phenyl-5,7-dihydroxy-3-beta-D-galactosyl-4H-chromen-4-one | 0.05 mM, 18.7% inhibition | Severe acute respiratory syndrome-related coronavirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03817 | - |
[4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] | mutant enzyme Q189A | Severe acute respiratory syndrome-related coronavirus | |
0.04938 | - |
[4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] | wild-type enzyme | Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] + H2O | - |
Severe acute respiratory syndrome-related coronavirus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3C-like protease | - |
Severe acute respiratory syndrome-related coronavirus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
[4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] | mutant enzyme Q189A | Severe acute respiratory syndrome-related coronavirus | |
0.16 | - |
[4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] | wild-type enzyme | Severe acute respiratory syndrome-related coronavirus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.024 | - |
- |
Severe acute respiratory syndrome-related coronavirus | 2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-L-fucosyl-4H-chromen-4-one | |
0.032 | - |
- |
Severe acute respiratory syndrome-related coronavirus | 2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-D-arabinosyl-4H-chromen-4-one | |
0.043 | - |
- |
Severe acute respiratory syndrome-related coronavirus | 2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-D-galactosyl-4H-chromen-4-one | |
0.049 | - |
- |
Severe acute respiratory syndrome-related coronavirus | 2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-D-glucosyl-4H-chromen-4-one | |
0.061 | - |
- |
Severe acute respiratory syndrome-related coronavirus | 2-(3',4'-dihydroxyphenyl)-5-hydroxy-3,7-di(beta-D-galactosyl)-4H-chromen-4-one |