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Literature summary for 3.4.22.69 extracted from

  • Chen, L.; Li, J.; Luo, C.; Liu, H.; Xu, W.; Chen, G.; Liew, O.W.; Zhu, W.; Puah, C.M.; Shen, X.; Jiang, H.
    Binding interaction of quercetin-3-beta -galactoside and its synthetic derivatives with SARS-CoV 3CLpro: Structure-activity relationship studies reveal salient pharmacophore features (2006), Bioorg. Med. Chem., 14, 8295-8306.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
Q189A kcat for the substrate [4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] is 1.14 fold lower than wild-type value, Km-value for the substrate [4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] is 1.3fold lower than the wild-type value Severe acute respiratory syndrome-related coronavirus

Inhibitors

Inhibitors Comment Organism Structure
2-(3',4'-dihydroxyphenyl)-3-beta-D-galactosyl-4H-chromen-4-one 0.05 mM, 30.1% inhibition Severe acute respiratory syndrome-related coronavirus
2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-D-arabinosyl-4H-chromen-4-one 0.05 mM, 49.4% inhibition Severe acute respiratory syndrome-related coronavirus
2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-D-galactosyl-4H-chromen-4-one 0.05 mM, 41.8% inhibition Severe acute respiratory syndrome-related coronavirus
2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-D-glucosyl-4H-chromen-4-one 0.05 mM, 57.5% inhibition Severe acute respiratory syndrome-related coronavirus
2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-L-fucosyl-4H-chromen-4-one 0.05 mM, 57.4% inhibition Severe acute respiratory syndrome-related coronavirus
2-(3',4'-dihydroxyphenyl)-5-hydroxy-3,7-di(beta-D-galactosyl)-4H-chromen-4-one 0.05 mM, 53.0% inhibition Severe acute respiratory syndrome-related coronavirus
2-phenyl-5,7-dihydroxy-3-beta-D-galactosyl-4H-chromen-4-one 0.05 mM, 18.7% inhibition Severe acute respiratory syndrome-related coronavirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03817
-
[4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] mutant enzyme Q189A Severe acute respiratory syndrome-related coronavirus
0.04938
-
[4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] wild-type enzyme Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] + H2O
-
Severe acute respiratory syndrome-related coronavirus ?
-
?

Synonyms

Synonyms Comment Organism
3C-like protease
-
Severe acute respiratory syndrome-related coronavirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
[4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] mutant enzyme Q189A Severe acute respiratory syndrome-related coronavirus
0.16
-
[4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] wild-type enzyme Severe acute respiratory syndrome-related coronavirus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.024
-
-
Severe acute respiratory syndrome-related coronavirus 2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-L-fucosyl-4H-chromen-4-one
0.032
-
-
Severe acute respiratory syndrome-related coronavirus 2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-D-arabinosyl-4H-chromen-4-one
0.043
-
-
Severe acute respiratory syndrome-related coronavirus 2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-D-galactosyl-4H-chromen-4-one
0.049
-
-
Severe acute respiratory syndrome-related coronavirus 2-(3',4'-dihydroxyphenyl)-5,7-dihydroxy-3-beta-D-glucosyl-4H-chromen-4-one
0.061
-
-
Severe acute respiratory syndrome-related coronavirus 2-(3',4'-dihydroxyphenyl)-5-hydroxy-3,7-di(beta-D-galactosyl)-4H-chromen-4-one