Application | Comment | Organism |
---|---|---|
analysis | developing a novel red-shifted fluorescence-based assay for 3CLpro and its application for identifying small-molecule anti-SARS agents from marine organisms | Severe acute respiratory syndrome-related coronavirus |
Cloned (Comment) | Organism |
---|---|
His-tagged SARS-CoV 3CL protease expressed in Escherichia coli | Severe acute respiratory syndrome-related coronavirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
esculetin-4-carboxylic acid ethyl ester | a novel class of anti-SARS agents from the tropical marine sponge Axinella corrugata | Severe acute respiratory syndrome-related coronavirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.146 | - |
o-aminobenzoyl-TSAVLQSGFRY(NO2)G | - |
Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged SARS-CoV 3CL protease | Severe acute respiratory syndrome-related coronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(CAL fluor red 610)-TSAVLQSGFRK(BHQ1) + H2O | - |
Severe acute respiratory syndrome-related coronavirus | (CAL fluor red 610)-TSAVLQ + SGFRK(BHQ1) | - |
? | |
o-aminobenzoyl-TSAVLQSGFRY(3-NO2)G + H2O | - |
Severe acute respiratory syndrome-related coronavirus | o-aminobenzoyl-TSAVLQ + SGFRY(3-NO2)G | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SARS-coronavirus 3CL protease | - |
Severe acute respiratory syndrome-related coronavirus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
o-aminobenzoyl-TSAVLQSGFRY(NO2)G | - |
Severe acute respiratory syndrome-related coronavirus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.046 | - |
- |
Severe acute respiratory syndrome-related coronavirus | esculetin-4-carboxylic acid ethyl ester |