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Literature summary for 3.4.22.69 extracted from

  • Solowiej, J.; Thomson, J.A.; Ryan, K.; Luo, C.; He, M.; Lou, J.; Murray, B.W.
    Steady-state and pre-steady-state kinetic evaluation of severe acute respiratory syndrome coronavirus (SARS-CoV) 3CLpro cysteine protease: development of an ion-pair model for catalysis (2008), Biochemistry, 47, 2617-2630.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state analysis of the solvent isotope effects on KM-value Severe acute respiratory syndrome-related coronavirus
0.045
-
[4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQSGFRKME-5-['-(aminoethyl)amino]-naphthalenesulfonic acid
-
Severe acute respiratory syndrome-related coronavirus
0.073
-
SITSAVLQ-p-nitrophenyl ester
-
Severe acute respiratory syndrome-related coronavirus
0.18
-
SITSAVLQ-p-nitroanilide
-
Severe acute respiratory syndrome-related coronavirus
0.6
-
SITSAVLQSGFRKMA
-
Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information a 3CLpro mechanism utilizes an electrostatic trigger to initiate the acylation reaction, a cysteine-histidine catalytic dyad ion pair, an enzyme-facilitated release of P1, and a general base catalyzed deacylation reaction Severe acute respiratory syndrome-related coronavirus ?
-
?
SITSAVLQ-p-nitroanilide + H2O
-
Severe acute respiratory syndrome-related coronavirus ?
-
?
SITSAVLQ-p-nitrophenyl ester + H2O
-
Severe acute respiratory syndrome-related coronavirus ?
-
?
SITSAVLQSGFRKMA + H2O
-
Severe acute respiratory syndrome-related coronavirus ?
-
?
[4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQSGFRKME-5-['-(aminoethyl)amino]-naphthalenesulfonic acid + H2O
-
Severe acute respiratory syndrome-related coronavirus ?
-
?
[4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQSGFRKME-5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid + H2O
-
Severe acute respiratory syndrome-related coronavirus [4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQ + SGFRKME-5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid
-
?
[4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQSGFRKME-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid] + H2O
-
Severe acute respiratory syndrome-related coronavirus ?
-
?

Synonyms

Synonyms Comment Organism
3cLpro
-
Severe acute respiratory syndrome-related coronavirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information steady-state analysis of the solvent isotope effects on kcat Severe acute respiratory syndrome-related coronavirus
0.6
-
SITSAVLQ-p-nitrophenyl ester
-
Severe acute respiratory syndrome-related coronavirus
0.86
-
SITSAVLQ-p-nitroanilide
-
Severe acute respiratory syndrome-related coronavirus
1.5
-
[4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQSGFRKME-5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid
-
Severe acute respiratory syndrome-related coronavirus
8.5
-
SITSAVLQSGFRKMA
-
Severe acute respiratory syndrome-related coronavirus