Cloned (Comment) | Organism |
---|---|
expressed in E. coli BL21 | Severe acute respiratory syndrome-related coronavirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTT | 80% of enzyme activity inhibited in the presence of 2.5 mM DTT | Severe acute respiratory syndrome-related coronavirus | |
NaCl | 80% of enzyme activity inhibited in the presence of 100 mM NaCl | Severe acute respiratory syndrome-related coronavirus | |
NP-40 | 80% of enzyme activity inhibited in the presence of 0.1% NP-40 | Severe acute respiratory syndrome-related coronavirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.306 | - |
(Ala-Arg-Leu-Gln-NH)2-rhodamine | rate of hydrolysis measured by change in absorbance at 496 nm | Severe acute respiratory syndrome-related coronavirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33760 | - |
determined by MALDI | Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | - |
virus infected Vero cells | - |
Purification (Comment) | Organism |
---|---|
strong cation column chromatography connected in series to a strong anion column chromatography | Severe acute respiratory syndrome-related coronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Ala-Arg-Leu-Gln-NH)2-rhodamine | - |
Severe acute respiratory syndrome-related coronavirus | rhodamine 110 + (Ala-Arg-Leu-Gln-NH)-rhodamine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Severe acute respiratory syndrome-related coronavirus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0153 | - |
(Ala-Arg-Leu-Gln-NH)2-rhodamine | rate of hydrolysis measured by change in absorbance at 496 nm | Severe acute respiratory syndrome-related coronavirus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Severe acute respiratory syndrome-related coronavirus |