Inhibitors | Comment | Organism | Structure |
---|---|---|---|
110 kDa calpastatin | - |
Bos taurus | |
70 kDa calpastatin | - |
Bos taurus | |
Calmidazolium | calpain-specific inhibitor | Bos taurus | |
leupeptin | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.459 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | endoplasmic reticulum lumen m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
0.461 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | endoplasmic reticulum membrane m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
2.1 | - |
succinyl-Leu-Tyr-7-amido-4-methylcoumarin | endoplasmic reticulum lumen m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
2.13 | - |
succinyl-Leu-Tyr-7-amido-4-methylcoumarin | endoplasmic reticulum membrane m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
4.66 | - |
succinyl-Leu-Met-7-amido-4-methylcoumarin | endoplasmic reticulum membrane m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
4.68 | - |
succinyl-Leu-Met-7-amido-4-methylcoumarin | endoplasmic reticulum lumen m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
8.11 | - |
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | endoplasmic reticulum membrane m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
8.12 | - |
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | endoplasmic reticulum lumen m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Bos taurus | 5783 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | optimum activity at 5 mM Ca2+ | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
8000 | - |
1 * 8000 + 1 * 28000, gel filtration, the endoplasmic reticulum membrane m-calpain containing 80000 Da large and 28000 Da small subunit is non-phosphorylated | Bos taurus |
28000 | - |
1 * 8000 + 1 * 28000, gel filtration, the endoplasmic reticulum membrane m-calpain containing 80000 Da large and 28000 Da small subunit is non-phosphorylated | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
IP3R1 + H2O | Bos taurus | in presence of Ca2þ, m-calpain cleaves IP3R1 in the endoplasmic lumen | ? | - |
? | |
NCX1 + H2O | Bos taurus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the endoplasmic reticulum lumen m-calpain containing only 80000 Da large subunit is phosphorylated | Bos taurus |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-cellulose column chromatography, phenyl-Sepharose column chromatography, DEAE-TSK column chromatography, and Reactive Red gel filtration | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pulmonary artery smooth muscle cell | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
endoplasmic reticulum membrane m-calpain shows 170 units/mg specific activity after 323fold purification, endoplasmic reticulum lumen m-calpain shows 324 units/mg specific activity after 463fold purification one unit is defined as the amount of the enzyme that causes a change of 1.0 in A750 for the trichloroacetic acid-soluble products after incubation of the enzyme in the assay mixture | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Bos taurus | ? | - |
? | |
IP3R1 + H2O | in presence of Ca2þ, m-calpain cleaves IP3R1 in the endoplasmic lumen | Bos taurus | ? | - |
? | |
NCX1 + H2O | - |
Bos taurus | ? | - |
? | |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? | |
succinyl-Leu-Met-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | succinyl-Leu-Met + 7-amino-4-methylcoumarin | - |
? | |
succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | succinyl-Leu-Tyr + 7-amino-4-methylcoumarin | - |
? | |
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | t-butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 8000 + 1 * 28000, gel filtration, the endoplasmic reticulum membrane m-calpain containing 80000 Da large and 28000 Da small subunit is non-phosphorylated | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
m-calpain | - |
Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | endoplasmic reticulum lumen m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
0.063 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | endoplasmic reticulum membrane m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
0.083 | - |
succinyl-Leu-Tyr-7-amido-4-methylcoumarin | endoplasmic reticulum lumen m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
0.084 | - |
succinyl-Leu-Tyr-7-amido-4-methylcoumarin | endoplasmic reticulum membrane m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
0.186 | - |
succinyl-Leu-Met-7-amido-4-methylcoumarin | endoplasmic reticulum membrane m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
0.189 | - |
succinyl-Leu-Met-7-amido-4-methylcoumarin | endoplasmic reticulum lumen m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
1.06 | - |
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | endoplasmic reticulum membrane m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
1.08 | - |
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | endoplasmic reticulum lumen m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bos taurus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bos taurus | isoelectric focusing | - |
4.5 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00000052 | - |
in 100 mM imidazole-HCl buffer (pH 7.5),5 mM cysteine and 5 mM CaCl2, at 30°C | Bos taurus | 110 kDa calpastatin | |
0.0000008 | - |
in 100 mM imidazole-HCl buffer (pH 7.5),5 mM cysteine and 5 mM CaCl2, at 30°C | Bos taurus | 70 kDa calpastatin |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0394 | - |
succinyl-Leu-Tyr-7-amido-4-methylcoumarin | endoplasmic reticulum membrane m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
0.0395 | - |
succinyl-Leu-Tyr-7-amido-4-methylcoumarin | endoplasmic reticulum lumen m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
0.0399 | - |
succinyl-Leu-Met-7-amido-4-methylcoumarin | endoplasmic reticulum membrane m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
0.0404 | - |
succinyl-Leu-Met-7-amido-4-methylcoumarin | endoplasmic reticulum lumen m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
0.131 | - |
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | endoplasmic reticulum membrane m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
0.133 | - |
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin | endoplasmic reticulum lumen m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
0.135 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | endoplasmic reticulum lumen m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus | |
0.137 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | endoplasmic reticulum membrane m-calpain, in 100 mM imidazole-HCl buffer (pH 7.5), 5 mM L-cysteine, 2.5 mM 2-mercaptoethanol, 5 mM CaCl2, and 4% (v/v) dimethyl sulfoxide, at 30°C | Bos taurus |