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Literature summary for 3.4.22.53 extracted from
- Limb-girdle muscular dystrophy type 2A can result from accelerated autoproteolytic inactivation of calpain 3 (2009), Biochemistry, 48, 3457-3467.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
- |
Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D346E | mutant with decreased enzymatic activity, increased rate of autoproteolytic degradation | Rattus norvegicus |
| D362K | mutant with decreased enzymatic activity, increased rate of autoproteolytic degradation | Rattus norvegicus |
| G423R | mutant with decreased enzymatic activity, increased rate of autoproteolytic degradation | Rattus norvegicus |
| additional information | used as a model for calpain 3 in combination with calpastatin-inhibited rat calpain 2 | Rattus norvegicus |
| additional information | used as a model for calpain 3 in combination with calpastatin-inhibited rat calpain 2 | Homo sapiens |
| R417W | mutant with decreased enzymatic activity, increased rate of autoproteolytic degradation | Rattus norvegicus |
| R628Q | mutant with decreased enzymatic activity | Rattus norvegicus |
| T344M | mutant with decreased enzymatic activity, increased rate of autoproteolytic degradation | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | initiation of autolysis of calpain 2 by adding of 1 mM CaCl2 | Rattus norvegicus |  |
| calpastatin | - |
Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
1 * 80000 + 1 * 21000, SDS-PAGE | Rattus norvegicus |
| 80000 | - |
1 * 80000 + 1 * 21000, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P04632 | - |
- |
| Rattus norvegicus | Q07009 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
initial activity as relative fluorescence units per s: 1.3 for wild type calpain 2, 0.84 for mutant G423R, 0.19 for mutant R628Q, 0.36 for mutant T344M, 0.7 for mutant D346E, 1.0 for mutant D362K | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FRET-based substrate PLFAER + H2O | 10 microM, pH 7.4, 1 mM of CaCl2 added to initiate the reaction | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 80000 + 1 * 21000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| calpain 2 | - |
Rattus norvegicus |
| calpain 2 | - |
Homo sapiens |
| human calpain 2 | - |
Homo sapiens |
| rat calpain 2 | - |
Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
1 mM CaCL2 added to start reaction | Rattus norvegicus |
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Release 2023.1 (January 2023)
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