Literature summary for 3.4.22.52 extracted from

  • Yoshimura, N.; Kikuchi, T.; Sasaki, T.; Kithara, A.; Hatanaka, M.; Murachi, T.
    Two distinct Ca2+ proteases (calpain I and calpain II) purified concurrently by the same method from rat kidney (1983), J. Biol. Chem., 258, 8883-8889.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens
Mn2+ activates Rattus norvegicus
Ba2+ activates Rattus norvegicus
Sr2+ activates Rattus norvegicus
Ca2+ half-maximal activation at 0.002 mM, full activity at 0.01 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
1 * 80000 + 1 * 25000, SDS-PAGE Rattus norvegicus
110000
-
gel filtration Rattus norvegicus
25000
-
1 * 80000 + 1 * 25000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens
additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
dimer
-
Homo sapiens
dimer 1 * 80000 + 1 * 25000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
10 min, more than 30% of the original activity is left Rattus norvegicus