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Literature summary for 3.4.22.16 extracted from
- Separation and properties of three forms of cathepsin H-like cysteine proteinase from rat spleen (1984), J. Biochem., 95, 477-484.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activates | Rattus norvegicus |  |
| cysteine | activates | Rattus norvegicus | |
| dithiothreitol | activates | Rattus norvegicus | |
| glutathione | activates | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate | 65-76% inhibition at 0.01 mg/ml, isoform-dependent | Rattus norvegicus | |
| iodoacetic acid | 71-77% inhibition at 0.5 mM, isoenzyme-dependent | Rattus norvegicus | |
| tosyl-Leu-CH2Cl | 17-35% inhibition at 0.1 mM, isoenzyme-dependent | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
gel filtration, isoform 3 | Rattus norvegicus |
| 26000 | - |
gel filtration, isoform 2 | Rattus norvegicus |
| 28000 | - |
gel filtration, isoform 1 | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
three isoforms | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spleen | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.714 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-N-benzoyl-DL-arginine-beta-naphthylamide + H2O | - |
Rattus norvegicus | alpha-N-benzoyl-DL-arginine + beta-naphthylamine | - |
ir | |
| Leu-2-naphthylamide + H2O | - |
Rattus norvegicus | Leu + 2-naphthylamine | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cathepsin H-like cysteine proteinase | - |
- |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
isoforms 1 and 3 | Rattus norvegicus |
| 7.5 | - |
isoform 2 | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Rattus norvegicus |
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