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Literature summary for 3.4.22.16 extracted from

  • Raghav, N.; Kamboj, R.C.; Parnami, S.; Singh, H.
    Physico-chemical properties of brain cathepsin H (1995), Indian J. Biochem. Biophys., 32, 279-285.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates, synergistic with EDTA Bos taurus
dithioerythritol activates, synergistic with EDTA Bos taurus
dithiothreitol activates, synergistic with EDTA Bos taurus
EDTA activates Bos taurus
glutathione activates, synergistic with EDTA Bos taurus
thioglycolic acid activates, synergistic with EDTA Bos taurus
thiol compounds activate Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl disulfide 96% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine Bos taurus
5,5'-dithiobis(2-nitrobenzoic acid) 95% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine Bos taurus
acetyl-(Ala)2-Pro-Ala-CH2Cl 100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine Bos taurus
acetyl-(Ala)2-Pro-Val-CH2Cl 100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine Bos taurus
acetyl-(Ala)4-CH2Cl 100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine Bos taurus
antipain 53% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine Bos taurus
bestatin 62% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus
decyl bromide 100% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine Bos taurus
ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate 90% inhibition at 0.001 mM, substrate Leu-2-N-naphthylamine Bos taurus
HgCl2 100% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine Bos taurus
iodoacetamide 91% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus
iodoacetic acid 100% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus
Leu-CH2Cl 92% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine Bos taurus
leupeptin 100% inhibition at 0.1 mM, substrate Leu-2-N-naphthylamine Bos taurus
MnSO4 64% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus
N-ethylmaleimide 98% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus
p-Hydroxymercuriphenylsulfonic acid 83% inhibition at 0.011 mM, substrate Leu-2-N-naphthylamine Bos taurus
Proteinase inhibitor rat liver Bos taurus
puromycin 20% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus
thiol-blocking agents
-
Bos taurus
tosyl-Leu-CH2Cl 63% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine Bos taurus
tosyl-Phe-CH2Cl 69% inhibition at 0.01 mM, substrate Leu-2-N-naphthylamine Bos taurus
ZnSO4 87% inhibition at 1 mM, substrate Leu-2-N-naphthylamine Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.128
-
Met-2-naphthylamide
-
Bos taurus
0.167
-
Leu-2-naphthylamide
-
Bos taurus
0.169
-
Arg-2-naphthylamide
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
286
-
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-N-benzoyl-DL-arginine-beta-naphthylamide + H2O
-
Bos taurus alpha-N-benzoyl-DL-arginine + beta-naphthylamine
-
ir
Arg-2-naphthylamide + H2O 97% activity of substrate Leu-2-N-naphthylamine Bos taurus Arg + 2-naphthylamine
-
ir
Leu-2-naphthylamide + H2O
-
Bos taurus Leu + 2-naphthylamine
-
ir
Met-2-naphthylamide + H2O 189% activity of substrate Leu-2-N-naphthylamine Bos taurus Met + 2-naphthylamine
-
ir

Subunits

Subunits Comment Organism
monomer SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50 substrate-dependent Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
inactivation above Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
115
-
Arg-2-naphthylamide
-
Bos taurus
122
-
Leu-2-naphthylamide
-
Bos taurus
219
-
Met-2-naphthylamide
-
Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.2 substrate-dependent Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 7.5
-
Bos taurus