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Literature summary for 3.4.22.14 extracted from

  • Baker, E.N.; Boland, M.J.; Calder, P.C.; Hardman, M.J.
    The specificity of actinidin and its relationship to the structure of the enzyme (1980), Biochim. Biophys. Acta, 616, 30-34.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
benzyloxycarbonyl-Lys p-nitrophenyl ester
-
Actinidia chinensis
0.044
-
butyloxycarbonyl-Phe-NH-CH2CN
-
Actinidia chinensis
0.12
-
benzyloxycarbonyl-Gly p-nitrophenyl ester
-
Actinidia chinensis
0.31
-
benzoyl-Gly p-nitrophenyl ester
-
Actinidia chinensis
1.8
-
Nalpha-benzoyl-L-Arg p-nitroanilide
-
Actinidia chinensis
21
-
benzoyl-Lys methyl ester benzoyl-L-Lys methyl ester Actinidia chinensis
61
-
benzoyl-Arg ethyl ester
-
Actinidia chinensis
78
-
acetyl-Lys methyl ester
-
Actinidia chinensis

Organism

Organism UniProt Comment Textmining
Actinidia chinensis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Gly p-nitrophenyl ester + H2O
-
Actinidia chinensis acetyl-Gly + 4-nitrophenol
-
?
acetyl-Gly-Lys methyl ester + H2O
-
Actinidia chinensis acetyl-Gly-Lys + methanol
-
?
acetyl-Lys methyl ester + H2O
-
Actinidia chinensis acetyl-Lys + methanol
-
?
benzoyl-Arg 4-nitroanilide + H2O
-
Actinidia chinensis benzoyl-Arg + 4-nitroaniline
-
?
benzoyl-Arg ethyl ester + H2O
-
Actinidia chinensis benzoyl-Arg + ethanol
-
?
benzoyl-Gly p-nitrophenyl ester + H2O
-
Actinidia chinensis benzoyl-Gly + p-nitrophenol
-
?
benzoyl-L-Lys methyl ester + H2O
-
Actinidia chinensis benzoyl-L-Lys + methanol
-
?
benzyloxycarbonyl 4-nitrophenyl ester + H2O
-
Actinidia chinensis benzyl formate + 4-nitrophenol
-
?
benzyloxycarbonyl-Gly p-nitrophenyl ester + H2O
-
Actinidia chinensis benzyloxycarbonyl-Gly + p-nitrophenol
-
?
Benzyloxycarbonyl-Lys methyl ester + H2O
-
Actinidia chinensis Benzyloxycarbonyl-Lys + methanol
-
?
benzyloxycarbonyl-Lys p-nitrophenyl ester + H2O
-
Actinidia chinensis benzyloxycarbonyl-Lys + 4-nitrophenol
-
?
Nalpha-benzoyl-L-Arg p-nitroanilide + H2O
-
Actinidia chinensis Nalpha-benzoyl-L-Arg + 4-nitroaniline
-
?
Tosyl-Arg methyl ester + H2O
-
Actinidia chinensis Tosyl-Arg + methanol
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.029
-
benzoyl-Arg 4-nitroanilide
-
Actinidia chinensis
0.7
-
acetyl-Lys methyl ester
-
Actinidia chinensis
3.4
-
benzyloxycarbonyl 4-nitrophenyl ester
-
Actinidia chinensis
4.5
-
benzoyl-Arg ethyl ester
-
Actinidia chinensis
5.8
-
benzoyl-Gly p-nitrophenyl ester
-
Actinidia chinensis
6
-
Benzyloxycarbonyl-Lys methyl ester
-
Actinidia chinensis
29
-
benzyloxycarbonyl-Lys p-nitrophenyl ester
-
Actinidia chinensis