Literature summary for 3.4.22.14 extracted from

Mostafaie, A.; Bidmeshkipour, A.; Shirvani, Z.; Mansouri, K.; Chalabi, M.
Kiwifruit actinidin: a proper new collagenase for isolation of cells from different tissues (2008), Appl. Biochem. Biotechnol., 144, 123-131.

Search for more literature for 3.4.22.14

Application

Application	Comment	Organism
molecular biology	actinidin compared with type II or IV collagenase isolates intact human umbilical vein endothelial cells, hepatocytes, and thymic epithelial cells with viability more than 90%	Actinidia deliciosa
synthesis	the enzyme can be used efficiently for hydrolysis of collagen and isolation of different cell populations from various solid tissues	Actinidia deliciosa

Organism

Organism	UniProt	Comment	Textmining
Actinidia deliciosa	-	-	-
Actinidia deliciosa	A5HII1	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Actinidia deliciosa
the enzyme fraction is precipitated from kiwifruit extract by 60% saturation of ammonium sulfate. The precipitate is redissolved in 50 mM citrate buffer (pH 5.5) and dialyzed overnight against this buffer. The dialyzate is loaded into a DEAE-Sepharose Fast Flow column, which pre-equilibrated with the same buffer. The adsorbed fractions are eluted with 0.0-1 M linear gradient of sodium chloride in the buffer	Actinidia deliciosa

Purification (Comment)

Organism

Actinidia deliciosa

the enzyme fraction is precipitated from kiwifruit extract by 60% saturation of ammonium sulfate. The precipitate is redissolved in 50 mM citrate buffer (pH 5.5) and dialyzed overnight against this buffer. The dialyzate is loaded into a DEAE-Sepharose Fast Flow column, which pre-equilibrated with the same buffer. The adsorbed fractions are eluted with 0.0-1 M linear gradient of sodium chloride in the buffer

Actinidia deliciosa

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	-	Actinidia deliciosa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
Collagen type I + H2O	actinidin can hydrolyze collagen types I and II at neutral and alkaline pH	Actinidia deliciosa	?	-	?
Collagen type I + H2O	the hydrolysis of types I and II collagen by actinidin under different pH conditions is monitored by SDS-PAGE: good digestion of type I collagen at pH 7 (phosphate buffer) or pH 8.5 (Tris-HCl buffer). In citrate buffer (pH 5.5), actinidin digests more than half of the collagen type I, but in acetate buffer (pH 4), the enzyme does not hydrolyze this substrate	Actinidia deliciosa	?	-	?
collagen type II + H2O	actinidin can hydrolyze collagen types I and II at neutral and alkaline pH	Actinidia deliciosa	?	-	?
collagen type II + H2O	the hydrolysis of types I and II collagen by actinidin under different pH conditions is monitored by SDS-PAGE: good digestion of type I collagen at pH 7 (phosphate buffer) or pH 8.5 (Tris-HCl buffer). In citrate buffer (pH 5.5), or acetate buffer (pH 4), the enzyme does not hydrolyze this substrate	Actinidia deliciosa	?	-	?
additional information	actinidin compared with type II or IV collagenase isolated from intact human umbilical vein endothelial cells, hepatocytes, and thymic epithelial cells with viability more than 90%	Actinidia deliciosa	?	-	?

Synonyms

Synonyms	Comment	Organism
actinidin	-	Actinidia deliciosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Actinidia deliciosa

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	8.5	efficient digestion of type I and type II collagen	Actinidia deliciosa