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Literature summary for 3.4.22.14 extracted from

  • Reid, J.D.; Hussain, S.; Bailey, T.S.; Sonkaria, S.; Sreedharan, S.K.; Thomas, E.W.; Resmini, M.; Brocklehurst, K.
    Isomerization of the uncomplexed actinidin molecule: kinetic accessibility of additional steps in enzyme catalysis provided by solvent perturbation (2004), Biochem. J., 378, 699-703.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
N-acetyl-L-Phe-Gly methyl ester above 0.25 mM, at 4.3% acetonitrile, 25°C, pH 5.3, parameter for pre-steady-state acylation phase Actinidia chinensis
0.543
-
N-acetyl-L-Phe-Gly methyl ester 25°C, pH 5.3, 8.3% acetonitrile parameter for pre-steady-state acylation phase Actinidia chinensis
0.893
-
N-acetyl-L-Phe-Gly methyl ester 25°C, pH 5.3, 16.7% acetonitrile, parameter for pre-steady-state acylation phase Actinidia chinensis

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile stopped-flow spectral analysis of increasing content of co-solvent acetonitrile on kinetics. With low acetonitrile content, plots of reaction are linear, increasing the acetonitrile content results in marked deviations of the plots from linearity with a rate minimum around [S]0 of 0.15 mM Actinidia chinensis
dimethyl sulfoxide comparison of effect on reaction kinetics with acetonitrile Actinidia chinensis

Organism

Organism UniProt Comment Textmining
Actinidia chinensis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-L-Phe-Gly methyl ester + H2O stopped-flow spectral analysis of increasing content of co-solvent acetonitrile on kinetics Actinidia chinensis ?
-
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