Crystallization (Comment) | Organism |
---|---|
crystal structures are determined of mature apo-SpeB at 1.06 A resolution from a crystal obtained in neutral pH conditions and high resolution crystal structures of SpeB in complex with the general proteinase inhibitor trans-epoxysuccinyl- L-leucylamido(4-guanidino)butane (E64) and in complex with the SpeB-specific N-terminal acetylated peptide aldehyde inhibitor acetyl-Ala-Gln-Ile-(S)-2,6-diaminohexanal | Streptococcus pyogenes |
Protein Variants | Comment | Organism |
---|---|---|
G378A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type | Streptococcus pyogenes |
G380A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type | Streptococcus pyogenes |
G381A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type | Streptococcus pyogenes |
G382A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type | Streptococcus pyogenes |
G384A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type | Streptococcus pyogenes |
G384D | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) 50% decreased compared to wild-type | Streptococcus pyogenes |
G385A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) minimally increased compared to wild-type and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) 3fold increased | Streptococcus pyogenes |
T379A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) slightly decreased compared to wild-type | Streptococcus pyogenes |
T379V | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) slightly decreased compared to wild-type | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-Ala-Gln-Ile-(S)-2,6-diaminohexanal | - |
Streptococcus pyogenes | |
trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane | - |
Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1178 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G384A, pH 7.4, 25°C | Streptococcus pyogenes | |
0.1387 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G380A, pH 7.4, 25°C | Streptococcus pyogenes | |
0.1832 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G384D, pH 7.4, 25°C | Streptococcus pyogenes | |
0.2023 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant T379V, pH 7.4, 25°C | Streptococcus pyogenes | |
0.2089 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G381A, pH 7.4, 25°C | Streptococcus pyogenes | |
0.2138 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant T379A, pH 7.4, 25°C | Streptococcus pyogenes | |
0.2163 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G378A, pH 7.4, 25°C | Streptococcus pyogenes | |
0.2239 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G382A, pH 7.4, 25°C | Streptococcus pyogenes | |
0.2466 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | wild-type, pH 7.4, 25°C | Streptococcus pyogenes | |
0.2477 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G385A, pH 7.4, 25°C | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Streptococcus pyogenes ATCC 10782 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin + H2O | - |
Streptococcus pyogenes | ? | - |
? | |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin + H2O | - |
Streptococcus pyogenes ATCC 10782 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SpeB | - |
Streptococcus pyogenes |
streptopain | - |
Streptococcus pyogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptococcus pyogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G384D, pH 7.4, 25°C | Streptococcus pyogenes | |
0.39 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G380A, pH 7.4, 25°C | Streptococcus pyogenes | |
0.4 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G378A, pH 7.4, 25°C | Streptococcus pyogenes | |
0.52 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G384A, pH 7.4, 25°C | Streptococcus pyogenes | |
0.57 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G382A, pH 7.4, 25°C | Streptococcus pyogenes | |
0.6 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G381A, pH 7.4, 25°C | Streptococcus pyogenes | |
0.62 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant T379V, pH 7.4, 25°C | Streptococcus pyogenes | |
0.63 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant T379A, pH 7.4, 25°C | Streptococcus pyogenes | |
0.69 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | wild-type, pH 7.4, 25°C | Streptococcus pyogenes | |
2.14 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G385A, pH 7.4, 25°C | Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Streptococcus pyogenes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G378A, pH 7.4, 25°C | Streptococcus pyogenes | |
1.9 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G384D, pH 7.4, 25°C | Streptococcus pyogenes | |
2.5 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G382A, pH 7.4, 25°C | Streptococcus pyogenes | |
2.8 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | wild-type, pH 7.4, 25°C | Streptococcus pyogenes | |
2.8 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G380A, pH 7.4, 25°C | Streptococcus pyogenes | |
2.9 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G381A, pH 7.4, 25°C | Streptococcus pyogenes | |
3 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant T379A, pH 7.4, 25°C | Streptococcus pyogenes | |
3 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant T379V, pH 7.4, 25°C | Streptococcus pyogenes | |
4.4 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G384A, pH 7.4, 25°C | Streptococcus pyogenes | |
8.6 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G385A, pH 7.4, 25°C | Streptococcus pyogenes |