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Literature summary for 3.4.22.10 extracted from
- Ultrahigh and high resolution structures and mutational analysis of monomeric Streptococcus pyogenes SpeB reveal a functional role for the glycine-rich C-terminal loop (2012), J. Biol. Chem., 287, 24412-24426.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structures are determined of mature apo-SpeB at 1.06 A resolution from a crystal obtained in neutral pH conditions and high resolution crystal structures of SpeB in complex with the general proteinase inhibitor trans-epoxysuccinyl- L-leucylamido(4-guanidino)butane (E64) and in complex with the SpeB-specific N-terminal acetylated peptide aldehyde inhibitor acetyl-Ala-Gln-Ile-(S)-2,6-diaminohexanal | Streptococcus pyogenes |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G378A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type | Streptococcus pyogenes |
| G380A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type | Streptococcus pyogenes |
| G381A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type | Streptococcus pyogenes |
| G382A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type | Streptococcus pyogenes |
| G384A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type | Streptococcus pyogenes |
| G384D | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) 50% decreased compared to wild-type | Streptococcus pyogenes |
| G385A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) minimally increased compared to wild-type and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) 3fold increased | Streptococcus pyogenes |
| T379A | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) slightly decreased compared to wild-type | Streptococcus pyogenes |
| T379V | Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) slightly decreased compared to wild-type | Streptococcus pyogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-Ala-Gln-Ile-(S)-2,6-diaminohexanal | - |
Streptococcus pyogenes |  |
| trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane | - |
Streptococcus pyogenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1178 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G384A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.1387 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G380A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.1832 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G384D, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.2023 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant T379V, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.2089 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G381A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.2138 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant T379A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.2163 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G378A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.2239 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G382A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.2466 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | wild-type, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.2477 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G385A, pH 7.4, 25°C | Streptococcus pyogenes | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pyogenes | - |
- |
- |
| Streptococcus pyogenes ATCC 10782 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin + H2O | - |
Streptococcus pyogenes | ? | - |
? | |
| acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin + H2O | - |
Streptococcus pyogenes ATCC 10782 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SpeB | - |
Streptococcus pyogenes |
| streptopain | - |
Streptococcus pyogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Streptococcus pyogenes |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G384D, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.39 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G380A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.4 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G378A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.52 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G384A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.57 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G382A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.6 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G381A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.62 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant T379V, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.63 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant T379A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 0.69 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | wild-type, pH 7.4, 25°C | Streptococcus pyogenes | |
| 2.14 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G385A, pH 7.4, 25°C | Streptococcus pyogenes | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Streptococcus pyogenes |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G378A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 1.9 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G384D, pH 7.4, 25°C | Streptococcus pyogenes | |
| 2.5 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G382A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 2.8 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | wild-type, pH 7.4, 25°C | Streptococcus pyogenes | |
| 2.8 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G380A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 2.9 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G381A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 3 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant T379A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 3 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant T379V, pH 7.4, 25°C | Streptococcus pyogenes | |
| 4.4 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G384A, pH 7.4, 25°C | Streptococcus pyogenes | |
| 8.6 | - |
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin | mutant G385A, pH 7.4, 25°C | Streptococcus pyogenes | |
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