Cloned (Comment) | Organism |
---|---|
genes speB and spi, nucleotide and amino acid sequence determination, analysis and comparison, co-expression of immature Spe B and Spi in Streptococcus pyogenes, Spi is similar to the pro-peptide of Spe B, overview, overexpression of His-tagged Spe B propeptide and SpeB mutant C47S in Escherichia coli | Streptococcus pyogenes |
Protein Variants | Comment | Organism |
---|---|---|
C47S | site-directed mutagenesis, inactive mutant | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Spe B pro-peptide | specific inhibition of the mature enzyme, secondary structure and SpeB binding analysis, inhibition modeling and mechanism, overview | Streptococcus pyogenes | |
Spi | specific enzyme pro-peptide analogue inhibitor, purification from Streptococcus pyogenes, secondary structure and SpeB binding analysis, inhibition modeling and mechanism, overview | Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Streptococcus pyogenes B220 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native mature Spe B by ammonium sulfate fractionation, dialysis, and ion exchange chromatography, recombinant His-tagged Spe B propeptide and SpeB mutant C47S from Escherichia coli | Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine serum albumin + H2O | - |
Streptococcus pyogenes | ? | - |
? | |
Bovine serum albumin + H2O | - |
Streptococcus pyogenes B220 | ? | - |
? | |
additional information | Spi and the Spe B pro-peptide both bind to mature Spe B, but are no substrates | Streptococcus pyogenes | ? | - |
? | |
additional information | Spi and the Spe B pro-peptide both bind to mature Spe B, but are no substrates | Streptococcus pyogenes B220 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary structure comparison of Spe B pro-peptide and Spi, overview | Streptococcus pyogenes |
Synonyms | Comment | Organism |
---|---|---|
SPE B protease | - |
Streptococcus pyogenes |
SpeB | - |
Streptococcus pyogenes |
streptococcal cysteine protease | - |
Streptococcus pyogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Streptococcus pyogenes |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics with Spi and Spe B pro-peptide | Streptococcus pyogenes |