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Literature summary for 3.4.22.10 extracted from

  • Kagawa, T.F.; Otoole, P.W.; Cooney, J.C.
    SpeB-Spi: a novel protease-inhibitor pair from Streptococcus pyogenes (2005), Mol. Microbiol., 57, 650-666.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes speB and spi, nucleotide and amino acid sequence determination, analysis and comparison, co-expression of immature Spe B and Spi in Streptococcus pyogenes, Spi is similar to the pro-peptide of Spe B, overview, overexpression of His-tagged Spe B propeptide and SpeB mutant C47S in Escherichia coli Streptococcus pyogenes

Protein Variants

Protein Variants Comment Organism
C47S site-directed mutagenesis, inactive mutant Streptococcus pyogenes

Inhibitors

Inhibitors Comment Organism Structure
Spe B pro-peptide specific inhibition of the mature enzyme, secondary structure and SpeB binding analysis, inhibition modeling and mechanism, overview Streptococcus pyogenes
Spi specific enzyme pro-peptide analogue inhibitor, purification from Streptococcus pyogenes, secondary structure and SpeB binding analysis, inhibition modeling and mechanism, overview Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Streptococcus pyogenes

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
-
-
Streptococcus pyogenes B220
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native mature Spe B by ammonium sulfate fractionation, dialysis, and ion exchange chromatography, recombinant His-tagged Spe B propeptide and SpeB mutant C47S from Escherichia coli Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Bovine serum albumin + H2O
-
Streptococcus pyogenes ?
-
?
Bovine serum albumin + H2O
-
Streptococcus pyogenes B220 ?
-
?
additional information Spi and the Spe B pro-peptide both bind to mature Spe B, but are no substrates Streptococcus pyogenes ?
-
?
additional information Spi and the Spe B pro-peptide both bind to mature Spe B, but are no substrates Streptococcus pyogenes B220 ?
-
?

Subunits

Subunits Comment Organism
More secondary structure comparison of Spe B pro-peptide and Spi, overview Streptococcus pyogenes

Synonyms

Synonyms Comment Organism
SPE B protease
-
Streptococcus pyogenes
SpeB
-
Streptococcus pyogenes
streptococcal cysteine protease
-
Streptococcus pyogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Streptococcus pyogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Streptococcus pyogenes

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics with Spi and Spe B pro-peptide Streptococcus pyogenes