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Literature summary for 3.4.22.1 extracted from

  • Yoshida, A.; Ohta, M.; Kuwahara, K.; Cao, M.J.; Hara, K.; Osatomi, K.
    Purification and characterization of cathepsin B from the muscle of horse mackerel Trachurus japonicus (2015), Mar. Drugs, 13, 6550-6565 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 2.0 mM, 1.75fold activation Trachurus japonicus
EDTA 1.0 mM, 1.2fold activation Trachurus japonicus

Application

Application Comment Organism
food industry the gel strength of modori gel is increased by suppression of cathepsin B activity using CA-074. Cathepsin B may cause modori phenomenon. Therefore, our results suggest that natural cysteine protease inhibitor, such as oryzacystatin derived from rice may apply to surimi-based product processing of horse mackerel to improve the quality of thermal gels Trachurus japonicus

Cloned(Commentary)

Cloned (Comment) Organism
-
Trachurus japonicus

Inhibitors

Inhibitors Comment Organism Structure
CA-074 0.01 mM, 97% loss of activity Trachurus japonicus
chymostatin 0.1 mM, 96% loss of activity Trachurus japonicus
E-64 0.01 mM, 99% loss of activity Trachurus japonicus
Pefabloc SC 1.0 mM, 11% loss of activity Trachurus japonicus

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
Trachurus japonicus 5764
-

Organism

Organism UniProt Comment Textmining
Trachurus japonicus A0A0P0YP15
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Trachurus japonicus

Purification (Commentary)

Purification (Comment) Organism
-
Trachurus japonicus

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Trachurus japonicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O 17% of the activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Trachurus japonicus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
Trachurus japonicus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
?
Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O 29% of the activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Trachurus japonicus Boc-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
?
Boc-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O 8% of the activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Trachurus japonicus Boc-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
?
additional information no activity with Arg-7-amido-4-methylcoumarin Trachurus japonicus ?
-
?
Suc-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O 6% of the activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Trachurus japonicus Suc-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
?

Subunits

Subunits Comment Organism
? x * 28000 (heavy chain), x * 60000 (light chain), SDS-PAGE Trachurus japonicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Trachurus japonicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 60 45°C: about 45% of maximal activity, 60°C: about 45% of maximal activity Trachurus japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Trachurus japonicus

pH Range

pH Minimum pH Maximum Comment Organism
4 6 pH 4.0: about 40% of maximal activity, pH 6.0: about 60% of maximal activity Trachurus japonicus