Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 2.0 mM, 1.75fold activation | Trachurus japonicus | |
EDTA | 1.0 mM, 1.2fold activation | Trachurus japonicus |
Application | Comment | Organism |
---|---|---|
food industry | the gel strength of modori gel is increased by suppression of cathepsin B activity using CA-074. Cathepsin B may cause modori phenomenon. Therefore, our results suggest that natural cysteine protease inhibitor, such as oryzacystatin derived from rice may apply to surimi-based product processing of horse mackerel to improve the quality of thermal gels | Trachurus japonicus |
Cloned (Comment) | Organism |
---|---|
- |
Trachurus japonicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CA-074 | 0.01 mM, 97% loss of activity | Trachurus japonicus | |
chymostatin | 0.1 mM, 96% loss of activity | Trachurus japonicus | |
E-64 | 0.01 mM, 99% loss of activity | Trachurus japonicus | |
Pefabloc SC | 1.0 mM, 11% loss of activity | Trachurus japonicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Trachurus japonicus | 5764 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trachurus japonicus | A0A0P0YP15 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Trachurus japonicus |
Purification (Comment) | Organism |
---|---|
- |
Trachurus japonicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Trachurus japonicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | 17% of the activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Trachurus japonicus | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Trachurus japonicus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O | 29% of the activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Trachurus japonicus | Boc-Phe-Ser-Arg + 7-amino-4-methylcoumarin | - |
? | |
Boc-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O | 8% of the activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Trachurus japonicus | Boc-Val-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
additional information | no activity with Arg-7-amido-4-methylcoumarin | Trachurus japonicus | ? | - |
? | |
Suc-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | 6% of the activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Trachurus japonicus | Suc-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000 (heavy chain), x * 60000 (light chain), SDS-PAGE | Trachurus japonicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Trachurus japonicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | 45°C: about 45% of maximal activity, 60°C: about 45% of maximal activity | Trachurus japonicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Trachurus japonicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | pH 4.0: about 40% of maximal activity, pH 6.0: about 60% of maximal activity | Trachurus japonicus |