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Literature summary for 3.4.22.1 extracted from

  • Niemer, M.; Mehofer, U.; Verdianz, M.; Porodko, A.; Schaehs, P.; Kracher, D.; Lenarcic, B.; Novinec, M.; Mach, L.
    Nicotiana benthamiana cathepsin B displays distinct enzymatic features which differ from its human relative and aleurain-like protease (2016), Biochimie, 122, 119-125 .
    View publication on PubMed
Search for more literature for 3.4.22.1

Application

Application Comment Organism
pharmacology Nicotiana benthamiana has recently emerged as a versatile expression platform for the rapid generation of recombinant biopharmaceuticals, but product yield and quality frequently suffer from unintended proteolysis. Targeted downregulation of cathepsin B can improve the performance of this plant-based expression platform Nicotiana benthamiana

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression of NbCathB in insect cells Nicotiana benthamiana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 Abz-GIVRAK-Dnp 37°C, pH 4.5 Nicotiana benthamiana
0.0014
-
 Abz-FRF(NO2)A 37°C, pH 4.5 Nicotiana benthamiana
0.048
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin 37°C, pH 4.5 Nicotiana benthamiana
0.099
-
 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin 37°C, pH 4.5 Nicotiana benthamiana

Organism

Organism UniProt Comment Textmining
Nicotiana benthamiana Q1HER6
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the theoretical molecular mass of pro-NbCathB is 36700 Da, whereas that of the mature protease is 28600 Da Nicotiana benthamiana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-FRF(NO2)A + H2O
-
 Nicotiana benthamiana ?
-
 ?
Abz-GIVRAK-Dnp + H2O
-
 Nicotiana benthamiana ?
-
 ?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Nicotiana benthamiana benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Nicotiana benthamiana benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
human anti-HIV-1 antibody + H2O NbCathB is far more efficient than aleurain-like protease in processing the human anti-HIV-1 antibody 2F5 into fragments Nicotiana benthamiana ?
-
 ?

Synonyms

Synonyms Comment Organism
NbCathB
-
 Nicotiana benthamiana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Nicotiana benthamiana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 Abz-GIVRAK-Dnp 37°C, pH 4.5 Nicotiana benthamiana
2.9
-
 Abz-FRF(NO2)A 37°C, pH 4.5 Nicotiana benthamiana
4.7
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin 37°C, pH 4.5 Nicotiana benthamiana
11
-
 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin 37°C, pH 4.5 Nicotiana benthamiana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 assay at Nicotiana benthamiana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
98
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin 37°C, pH 4.5 Nicotiana benthamiana
111
-
 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin 37°C, pH 4.5 Nicotiana benthamiana
116
-
 Abz-GIVRAK-Dnp 37°C, pH 4.5 Nicotiana benthamiana
2037
-
 Abz-FRF(NO2)A 37°C, pH 4.5 Nicotiana benthamiana