Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | cathepsin B is activated at 22°C for 5 min in 100 mM sodium acetate, 1 mM DTT, 2 mM EDTA, 4 mM cysteine, pH 5.5 | Angiostrongylus cantonensis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Angiostrongylus cantonensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cystatin | the enzyme retains 10% and 5% of its initial activity, after 30 min of incubation at 37°C, in the presence of 75 and 100 nM recombinant cystatin, respectively | Angiostrongylus cantonensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Angiostrongylus cantonensis |
40850 | - |
x * 40850, calculated from amino acid sequence | Angiostrongylus cantonensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ovalbumin + H2O | Angiostrongylus cantonensis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Angiostrongylus cantonensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon affinity column chromatography | Angiostrongylus cantonensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | - |
Angiostrongylus cantonensis | - |
larva | the enzyme is expressed significantly higher in the fourth-stage larvae and the fifth-stage larvae than that in the third-stage larvae and adult worms | Angiostrongylus cantonensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Angiostrongylus cantonensis | N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
ovalbumin + H2O | - |
Angiostrongylus cantonensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Angiostrongylus cantonensis |
? | x * 40850, calculated from amino acid sequence | Angiostrongylus cantonensis |
Synonyms | Comment | Organism |
---|---|---|
CPB | - |
Angiostrongylus cantonensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Angiostrongylus cantonensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
17 | 57 | the activity is relatively stable at 17°C and 27°C, retaining about 95% and 98% activity, respectively. The relative activities at 47°C and 57°C are 31% and 13% of that at 37°C, respectively | Angiostrongylus cantonensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
99 | - |
the activity drops dramatically within the first 30 min and 70% activity is lost after 30 min of incubation at 99°C | Angiostrongylus cantonensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Angiostrongylus cantonensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | there is 60% activity at pH 4.0. The activity decreases significantly below pH 4.0 or above pH 5.0, and 5% activity is detected at pH 6.0 | Angiostrongylus cantonensis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.06 | - |
at pH 5.0 and 37°C | Angiostrongylus cantonensis | cystatin |