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Literature summary for 3.4.22.1 extracted from

  • Yao, X.; Zhang, J.; Sun, J.; Liu, B.
    Recombinant expression, characterization and expressional analysis of clam Meretrix meretrix cathepsin B, an enzyme involved in nutrient digestion (2011), Mol. Biol. Rep., 38, 1861-1868.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Meretrix meretrix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00611
-
 Nalpha-carbobenzoxy-L-Arg-L-Arg-7-amido-4-trifluoromethylcoumarin recombinant enzyme, at pH 7.3 and 37°C Meretrix meretrix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 2 * 37000, SDS-PAGE Meretrix meretrix
63000
-
 LC-ESI mass spectrometry Meretrix meretrix

Organism

Organism UniProt Comment Textmining
Meretrix meretrix B6CPA2
-
-

Purification (Commentary)

Purification (Comment) Organism
GSTrap FF column chromatography Meretrix meretrix

Source Tissue

Source Tissue Comment Organism Textmining
adductor muscle
-
 Meretrix meretrix
-
digestive gland highest expression Meretrix meretrix
-
gill moderate expression Meretrix meretrix
-
mantle moderate expression Meretrix meretrix
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
74.4
-
 recombinant enzyme, at pH 7.3 and 37°C Meretrix meretrix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-trifluoromethylcoumarin + H2O
-
 Meretrix meretrix Nalpha-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-trifluoromethylcoumarin
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 37000, SDS-PAGE Meretrix meretrix

Synonyms

Synonyms Comment Organism
MmeCB
-
 Meretrix meretrix

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
277.6
-
 Nalpha-carbobenzoxy-L-Arg-L-Arg-7-amido-4-trifluoromethylcoumarin recombinant enzyme, at pH 7.3 and 37°C Meretrix meretrix

Expression

Organism Comment Expression
Meretrix meretrix starvation causes a slight increase in MmeCB protein synthesis in the digestive gland, while refeeding after starvation causes an apparent increase in MmeCB synthesis in digestive gland, gill and mantle. MmeCB mRNA in digestive gland is 3.5fold up-regulated by starvation up

General Information

General Information Comment Organism
physiological function cathepsin B is involved in the nutrient digestion of Meretrix meretrix Meretrix meretrix

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
45.4
-
 Nalpha-carbobenzoxy-L-Arg-L-Arg-7-amido-4-trifluoromethylcoumarin recombinant enzyme, at pH 7.3 and 37°C Meretrix meretrix