Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2A2 monoclonal antibody | binds to the epitope EPGYSP sequence, i.e. in the nonapeptide CIAEPGYSP, that is located between amino acid residues 133-138 of cathepsin B in the proximity of the occluding loop, surface plasmon resonance analysis, overview. Binding of 2A2 monoclonal antibody to the cathepsin B/cystatin C complex causes the dissociation of cystatin C from the complex, and binding of the antibody induces a conformational change in cathepsin B, stabilizing its exopeptidase conformation and thus disabling its harmful action associated with its endopeptidase activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
BODIPY FL casein + H2O | - |
Homo sapiens | ? | - |
? | |
DQ-collagen IV + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | cathepsin B is a cysteine protease with both endopeptidase and exopeptidase activity | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
exopeptidase activity assay at | Homo sapiens |
6 | - |
endopeptidase activity assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
702 | - |
pH 6.0, 37°C, versus substrate DQ-collagen IV | Homo sapiens | 2A2 monoclonal antibody | |
761 | - |
pH 6.0, 37°C, versus substrate BODIPY FL casein | Homo sapiens | 2A2 monoclonal antibody |
General Information | Comment | Organism |
---|---|---|
physiological function | the endopeptidase activity of cathepsin B is associated with the degradation of the extracellular matrix proteins, which is a process required for tumor cell invasion and metastasis, the activity can be inhibited by the 2A2 monoclonal antibody | Homo sapiens |