Cloned (Comment) | Organism |
---|---|
expression in CABA I ovarian carcinoma cells, cathepsin B expression analysis by real-time PCR | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | cathepsin B gene silencing by using cathepsin B small interfering RNA, siRNA, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CA-074 | a selective cell-impermeable cathepsin B inhibitor | Homo sapiens | |
CA-074Me | a selective cell-permeable cathepsin B inhibitor | Homo sapiens | |
E-64 | broad-spectrum cysteine protease inhibitor, complete inhibition | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vesicle | tumor-shed microvesicle | Homo sapiens | 31982 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
x * 15000, form I, SDS-PAGE, x * 20000, form II, SDS-PAGE, x * 30000, form III, SDS-PAGE | Homo sapiens |
20000 | - |
x * 15000, form I, SDS-PAGE, x * 20000, form II, SDS-PAGE, x * 30000, form III, SDS-PAGE | Homo sapiens |
30000 | - |
x * 15000, form I, SDS-PAGE, x * 20000, form II, SDS-PAGE, x * 30000, form III, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | Homo sapiens | - |
? | - |
? | |
additional information | Homo sapiens | cysteine cathepsins play a critical role in the biologic activity of tumor-shed vesicles at acidic pH, but not at physiological pH, endothelial cell invasiveness is increased by tumor-shed vesicles exposed to acidic pH, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CABA I cell | - |
Homo sapiens | - |
fibrosarcoma cell | - |
Homo sapiens | - |
HT-1080 cell | - |
Homo sapiens | - |
ovary carcinoma cell | - |
Homo sapiens | - |
umbilical vein endothelial cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O | a fluorogenic substrate of Na-CP-3 | Homo sapiens | carbobenzoxy-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | cysteine cathepsins play a critical role in the biologic activity of tumor-shed vesicles at acidic pH, but not at physiological pH, endothelial cell invasiveness is increased by tumor-shed vesicles exposed to acidic pH, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 15000, form I, SDS-PAGE, x * 20000, form II, SDS-PAGE, x * 30000, form III, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
assay at | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | 7.4 | activity measurement range, vesicle-associated cathepsin B activity is increased 1300fold at acidic pH values compared to physiological pH 7.4, cathepsin B activity in cell extract is increased 33fold at pH 5.6 versus pH 7.4 | Homo sapiens |