Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.1 extracted from

  • Giusti, I.; D'Ascenzo, S.; Millimaggi, D.; Taraboletti, G.; Carta, G.; Franceschini, N.; Pavan, A.; Dolo, V.
    Cathepsin B mediates the pH-dependent proinvasive activity of tumor-shed microvesicles (2008), Neoplasia, 10, 481-488.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in CABA I ovarian carcinoma cells, cathepsin B expression analysis by real-time PCR Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information cathepsin B gene silencing by using cathepsin B small interfering RNA, siRNA, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
CA-074 a selective cell-impermeable cathepsin B inhibitor Homo sapiens
CA-074Me a selective cell-permeable cathepsin B inhibitor Homo sapiens
E-64 broad-spectrum cysteine protease inhibitor, complete inhibition Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
vesicle tumor-shed microvesicle Homo sapiens 31982
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
x * 15000, form I, SDS-PAGE, x * 20000, form II, SDS-PAGE, x * 30000, form III, SDS-PAGE Homo sapiens
20000
-
x * 15000, form I, SDS-PAGE, x * 20000, form II, SDS-PAGE, x * 30000, form III, SDS-PAGE Homo sapiens
30000
-
x * 15000, form I, SDS-PAGE, x * 20000, form II, SDS-PAGE, x * 30000, form III, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Gelatin + H2O Homo sapiens
-
?
-
?
additional information Homo sapiens cysteine cathepsins play a critical role in the biologic activity of tumor-shed vesicles at acidic pH, but not at physiological pH, endothelial cell invasiveness is increased by tumor-shed vesicles exposed to acidic pH, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
CABA I cell
-
Homo sapiens
-
fibrosarcoma cell
-
Homo sapiens
-
HT-1080 cell
-
Homo sapiens
-
ovary carcinoma cell
-
Homo sapiens
-
umbilical vein endothelial cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O a fluorogenic substrate of Na-CP-3 Homo sapiens carbobenzoxy-Arg-Arg + 7-amino-4-methylcoumarin
-
?
Gelatin + H2O
-
Homo sapiens ?
-
?
additional information cysteine cathepsins play a critical role in the biologic activity of tumor-shed vesicles at acidic pH, but not at physiological pH, endothelial cell invasiveness is increased by tumor-shed vesicles exposed to acidic pH, overview Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 15000, form I, SDS-PAGE, x * 20000, form II, SDS-PAGE, x * 30000, form III, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
assay at Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.8 7.4 activity measurement range, vesicle-associated cathepsin B activity is increased 1300fold at acidic pH values compared to physiological pH 7.4, cathepsin B activity in cell extract is increased 33fold at pH 5.6 versus pH 7.4 Homo sapiens