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Literature summary for 3.4.22.1 extracted from

  • Cavallo-Medved, D.; Rudy, D.; Blum, G.; Bogyo, M.; Caglic, D.; Sloane, B.F.
    Live-cell imaging demonstrates extracellular matrix degradation in association with active cathepsin B in caveolae of endothelial cells during tube formation (2009), Exp. Cell Res., 315, 1234-1246.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.1

Activating Compound

Activating Compound Comment Organism Structure
additional information activator buffer contains 5 mM EDTA, 10 mM DTT, pH 5.2 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
caveola of the lysosomal fraction, co-localization of active cathepsin B and its cell surface binding partners, S100A/p11 of the annexin II heterotetramer, to caveolae of human umbilical vein endothelial cells and colorectal carcinoma cells Homo sapiens 5901
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 31000, single chain cathepsin B, SDS-PAGE, x * 25000-26000, heavy chain of double chain cathepsin B, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen IV + H2O Homo sapiens
-
 ?
-
 ?
Gelatin + H2O Homo sapiens
-
 ?
-
 ?
additional information Homo sapiens the enzyme is involved in extracellular matrix degradation in caveolae of endothelial cells during tube formation, overview. Cathepsin B regulates both pro- and anti-angiogenic factors and may be a contributor to the angiogenic switch of endothelial cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from caveolae of human umbilical vein endothelial cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
colonic cancer cell
-
 Homo sapiens
-
colorectal cancer cell
-
 Homo sapiens
-
endothelial cell umbilical vein endothelial cells, caveolae Homo sapiens
-
HCT-116 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Collagen IV + H2O
-
 Homo sapiens ?
-
 ?
Collagen IV + H2O quenched fluorescent protein Homo sapiens ?
-
 ?
Gelatin + H2O
-
 Homo sapiens ?
-
 ?
Gelatin + H2O quenched fluorescent protein Homo sapiens ?
-
 ?
additional information the enzyme is involved in extracellular matrix degradation in caveolae of endothelial cells during tube formation, overview. Cathepsin B regulates both pro- and anti-angiogenic factors and may be a contributor to the angiogenic switch of endothelial cells Homo sapiens ?
-
 ?
N-alpha-benzyloxycarbonyl-L-arginyl-L-arginine-4-methyl-7-coumarylamide + H2O
-
 Homo sapiens N-alpha-benzyloxycarbonyl-L-arginyl-L-arginine + 4-methyl-7-coumarylamine
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, single chain cathepsin B, SDS-PAGE, x * 25000-26000, heavy chain of double chain cathepsin B, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
cysteine cathepsin
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Homo sapiens