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Literature summary for 3.4.22.1 extracted from

  • Caglic, D.; Kosec, G.; Bojic, L.; Reinheckel, T.; Turk, V.; Turk, B.
    Murine and human cathepsin B exhibit similar properties: possible implications for drug discovery (2009), Biol. Chem., 390, 175-179.
    View publication on PubMed

Application

Application Comment Organism
drug development cathepsin B could be a drug target for several pathologies, based mainly on its role in cancer progression and osteoarthritis in humans Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in inclusion bodies in Escherichia coli Homo sapiens
expression in inclusion bodies in Escherichia coli, cathepsin B gene is located on chromosome 14 of the mouse genome Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of cathepsin B transgenic mouse models for investigation of human pathologies Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
CA-074 irreversible cathepsin B inhibitor Homo sapiens
CA-074 irreversible cathepsin B inhibitor Mus musculus
chicken cystatin
-
Homo sapiens
chicken cystatin
-
Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin pH 6.0, 37°C, recombinant enzyme Mus musculus
0.075
-
Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin pH 6.0, 37°C, recombinant enzyme Homo sapiens
0.212
-
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin pH 6.0, 37°C, recombinant enzyme Homo sapiens
0.226
-
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin pH 6.0, 37°C, recombinant enzyme Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens disturbances in the regulation of the intracellular activities and uncontrolled release of cathepsins from lysosomes have been implicated in many disease states, such as osteoporosis, inflammatory diseases, and tumor progression ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P07858
-
-
Mus musculus P10605
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant renatured enzyme expressed in inclusion bodies in Escherichia coli Homo sapiens
recombinant renatured enzyme expressed in inclusion bodies in Escherichia coli Mus musculus

Renatured (Commentary)

Renatured (Comment) Organism
recombinant enzyme from inclusion bodies from Escherichia coli Homo sapiens
recombinant enzyme from inclusion bodies from Escherichia coli Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast embryonic Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens
additional information
-
-
Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information disturbances in the regulation of the intracellular activities and uncontrolled release of cathepsins from lysosomes have been implicated in many disease states, such as osteoporosis, inflammatory diseases, and tumor progression Homo sapiens ?
-
?
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Homo sapiens Nalpha-benzoyl-Arg-Arg + 7-amino-4-methylcoumarin
-
?
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Mus musculus Nalpha-benzoyl-Arg-Arg + 7-amino-4-methylcoumarin
-
?
Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
Homo sapiens Nalpha-benzoyl-Phe-Arg + 7-amino-4-methylcoumarin
-
?
Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
Mus musculus Nalpha-benzoyl-Phe-Arg + 7-amino-4-methylcoumarin
-
?

Subunits

Subunits Comment Organism
More structure comparison to the murine enzyme Homo sapiens
More three-dimensional enzyme structure, modeling, structure comparison to the human enzyme, overview Mus musculus

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the group of cysteine proteases Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens
37
-
assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
34
-
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin pH 6.0, 37°C, recombinant enzyme Mus musculus
67
-
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin pH 6.0, 37°C, recombinant enzyme Homo sapiens
86
-
Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin pH 6.0, 37°C, recombinant enzyme Mus musculus
115
-
Nalpha-benzoyl-Phe-Arg-7-amido-4-methylcoumarin pH 6.0, 37°C, recombinant enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Homo sapiens
6
-
assay at Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000003
-
chicken cystatin
-
Homo sapiens
0.00000125
-
chicken cystatin
-
Mus musculus