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Literature summary for 3.4.22.1 extracted from

  • Hoang, V.L.; Li, Y.; Kim, S.K.
    Cathepsin B inhibitory activities of phthalates isolated from a marine Pseudomonas strain (2008), Bioorg. Med. Chem. Lett., 18, 2083-2088.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in murine melanoma cell line B16-F10 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
CA030
-
Homo sapiens
di-(2-ethylhexyl)phthalate non-competitive inhibitor Homo sapiens
dibutyl phthalate non-competitive inhibitor Homo sapiens
E-64
-
Homo sapiens
leupeptin
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
Homo sapiens 5764
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-L-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Homo sapiens benzyloxycarbonyl-L-Arg-Arg + 7-amino-4-methylcoumarin
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.42
-
di-(2-ethylhexyl)phthalate wild type enzyme, in 0.4 M sodium potassium phosphate buffer (pH 6.0) containing 8 mM dithiothreitol and 4 mM EDTA, at 37°C Homo sapiens
0.64
-
dibutyl phthalate wild type enzyme, in 0.4 M sodium potassium phosphate buffer (pH 6.0) containing 8 mM dithiothreitol and 4 mM EDTA, at 37°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.14
-
recombinant enzyme, in Hank's balanced salt solution containing 2 mM L-cysteine, pH 7.0, at 37°C Homo sapiens di-(2-ethylhexyl)phthalate
0.23
-
recombinant enzyme, in Hank's balanced salt solution containing 2 mM L-cysteine, pH 7.0, at 37°C Homo sapiens dibutyl phthalate
0.38
-
wild type enzyme, in 0.4 M sodium potassium phosphate buffer (pH 6.0) containing 8 mM dithiothreitol and 4 mM EDTA, at 37°C Homo sapiens di-(2-ethylhexyl)phthalate
0.42
-
wild type enzyme, in 0.4 M sodium potassium phosphate buffer (pH 6.0) containing 8 mM dithiothreitol and 4 mM EDTA, at 37°C Homo sapiens dibutyl phthalate