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Literature summary for 3.4.22.1 extracted from

  • Ruzza, P.; Quintieri, L.; Osler, A.; Calderan, A.; Biondi, B.; Floreani, M.; Guiotto, A.; Borin, G.
    Fluorescent, internally quenched, peptides for exploring the pH-dependent substrate specificity of cathepsin B (2006), J. Pept. Sci., 12, 455-461.
    View publication on PubMed

Application

Application Comment Organism
drug development peptides containing Ala or Phe at positions 6 are good substrates for the enzyme at both pH 5.0 and 7.4. Those containing Glu, Asp, Lys or Val are not cleaved at all by cathepsin B at pH 7.4 and are poorly hydrolized at pH 5.0. These findings provide new information for the rational design of cathepsin B-activated peptide-containing anticancer drugs Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bos taurus
-
-
lysosome acidic Bos taurus 5764
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Organism

Organism UniProt Comment Textmining
Bos taurus P07688
-
-

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Z-Phe-Arg-4-methylcoumarin-7-amide + H2O
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Bos taurus Z-Phe-Arg + 7-amino-4-methylcoumarin
-
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Synonyms

Synonyms Comment Organism
cathepsin B
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Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4 5.5 attack from C-terminus Bos taurus
7.4
-
endoproteolytic attack Bos taurus