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Literature summary for 3.4.22.1 extracted from

  • Almeida, P.C.; Nantes, I.L.; Chagas, J.R.; Rizzi, C.C.; Faljoni-Alario, A.; Carmona, E.; Juliano, L.; Nader, H.B.; Tersariol, I.L.
    Cathepsin B activity regulation. Heparin-like glycosaminogylcans protect human cathepsin B from alkaline pH-induced inactivation (2001), J. Biol. Chem., 276, 944-951.
    View publication on PubMed
Search for more literature for 3.4.22.1

Activating Compound

Activating Compound Comment Organism Structure
heparin at pH 8.0, heparin increases the half-life of wild type enzyme from 84 sec to 433 sec Homo sapiens

Protein Variants

Protein Variants Comment Organism
H110A 4fold decrease in pH stability compared to wild type, inhibition by heparin similar to wild type Homo sapiens
H111A presence of heparin does not affect Km-value, heparin is not inhibitory Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
heparin inhibition is strongly dependent on pH, no inhibition above pH 7.0 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0125
-
 o-aminobenzoyl-L-Phe-L-Arg-L-Lys-epsilon-N-2,4-dinitrophenylamide pH 6.0, 25°C, wild-type enzyme Homo sapiens
0.013
-
 o-aminobenzoyl-L-Phe-L-Arg-L-Lys-epsilon-N-2,4-dinitrophenylamide pH 6.0, 25°C, mutant H110A Homo sapiens
0.014
-
 o-aminobenzoyl-L-Phe-L-Arg-L-Lys-epsilon-N-2,4-dinitrophenylamide pH 6.0, 25°C, mutant H111A in presence of heparin Homo sapiens
0.016
-
 o-aminobenzoyl-L-Phe-L-Arg-L-Lys-epsilon-N-2,4-dinitrophenylamide pH 6.0, 25°C, mutant H111A Homo sapiens
0.043
-
 o-aminobenzoyl-L-Phe-L-Arg-L-Lys-epsilon-N-2,4-dinitrophenylamide pH 6.0, 25°C, wild-type enzyme in presence of heparin Homo sapiens
0.047
-
 o-aminobenzoyl-L-Phe-L-Arg-L-Lys-epsilon-N-2,4-dinitrophenylamide pH 6.0, 25°C, mutant H110A in presence of heparin Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
o-aminobenzoyl-L-Phe-L-Arg-L-Lys-epsilon-N-2,4-dinitrophenylamide + H2O
-
 Homo sapiens ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 substrate-dependent Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
8
-
 half-life of wild type enzyme 84 s, mutant H110A 22 s, mutant H111A 75 s Homo sapiens