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Literature summary for 3.4.22.1 extracted from

  • Inaba, T.; Shindo, N.; Fujii, M.
    Purification of cathepsin B from squid liver (1976), Agric. Biol. Chem., 40, 1159-1165.
No PubMed abstract available
Search for more literature for 3.4.22.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.25
-
 benzoyl-DL-Arg-beta-naphthylamide
-
 Dorytheuthis bleekeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13600
-
 gel filtration Dorytheuthis bleekeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Proteins + H2O Dorytheuthis bleekeri
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Dorytheuthis bleekeri
-
 squid
-

Purification (Commentary)

Purification (Comment) Organism
-
 Dorytheuthis bleekeri

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Dorytheuthis bleekeri
-

Storage Stability

Storage Stability Organism
-20°C, 1 month, stable Dorytheuthis bleekeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-DL-Arg-beta-naphthylamide + H2O
-
 Dorytheuthis bleekeri benzoyl-DL-Arg + beta-naphthylamine
-
 ?
benzoyl-DL-argininamide + H2O
-
 Dorytheuthis bleekeri benzoyl-DL-arginine + NH3
-
 ?
N,N-dimethyl hemoglobin + H2O
-
 Dorytheuthis bleekeri hydrolyzed N,N-dimethyl hemoglobin
-
 ?
Proteins + H2O
-
 Dorytheuthis bleekeri ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 stable for 60 min, activity rapidly lost at higher temperatures Dorytheuthis bleekeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2 4.7
-
 Dorytheuthis bleekeri