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Literature summary for 3.4.22.1 extracted from
- The lysosomal proteinase of Artemia. Purification and characterization (1982), Eur. J. Biochem., 124, 357-362.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | activates | Artemia sp. |  |
| EDTA | no effect at 0.5 mM | Artemia sp. | |
| thiol compounds | required for full activity | Artemia sp. | |
General Stability
| General Stability | Organism |
|---|---|
| glycerol stabilizes | Artemia sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antipain | - |
Artemia sp. | |
| Cu2+ | - |
Artemia sp. | |
| H2O2 | - |
Artemia sp. | |
| iodoacetic acid | - |
Artemia sp. | |
| KI | - |
Artemia sp. | |
| L-3-carboxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane | E-64 | Artemia sp. | |
| leupeptin | - |
Artemia sp. | |
| N-ethylmaleimide | no inhibition at 1 mM | Artemia sp. | |
| p-chloromercuriphenyl sulfonic acid | - |
Artemia sp. | |
| p-hydroxymercuribenzoate | - |
Artemia sp. | |
| phenylmethanesulfonyl fluoride | no inhibition at 10 microM | Artemia sp. | |
| Zn2+ | - |
Artemia sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
protamine | - |
Artemia sp. | |
| 0.02 | - |
benzyloxycarbonyl-Arg-Arg-4-methoxy-beta-naphthylamide | - |
Artemia sp. | |
| 0.13 | - |
D-Pro-Phe-Arg-p-nitroanilide | - |
Artemia sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
filtration, SDS-PAGE | Artemia sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Proteins + H2O | Artemia sp. | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Artemia sp. | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | glycoprotein | Artemia sp. |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Artemia sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gastrula | dormant gastrula, cysts | Artemia sp. | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Arg-Arg-4-methoxy-beta-naphthylamide + H2O | - |
Artemia sp. | benzyloxycarbonyl-Arg-Arg + 4-methoxy-beta-naphthylamine | - |
? | |
| Bovine serum albumin + H2O | - |
Artemia sp. | Hydrolyzed bovine serum albumin | - |
? | |
| D-Pro-Phe-Arg-p-nitroanilide + H2O | - |
Artemia sp. | D-Pro-Phe-Arg + p-nitroaniline | - |
? | |
| D-Val-Leu-Lys-p-nitroanilide + H2O | - |
Artemia sp. | D-Val-Leu-Lys + p-nitroaniline | - |
? | |
| Protamine + H2O | - |
Artemia sp. | Hydrolyzed protamine | - |
? | |
| Proteins + H2O | - |
Artemia sp. | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1*68000, gel filtration, SDS-PAGE | Artemia sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Artemia sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.3 | 7 | stable, optimum stability at pH 7.0 | Artemia sp. |
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