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Literature summary for 3.4.22.1 extracted from

  • Takahashi, T.; Yonezawa, S.; Dehdarani, A.H.; Tang, J.
    Comparative studies of two cathepsin B isozymes from porcine spleen. Isolation, polypeptide chain arrangements, and enzyme specificity (1986), J. Biol. Chem., 261, 9368-9374.
    View publication on PubMed
Search for more literature for 3.4.22.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
 benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-beta-naphthylamide isoenzyme II Sus scrofa
0.07
-
 benzoyl-Phe-Val-Arg-p-nitroanilide isoenzyme I Sus scrofa
0.072
-
 N-t-butyloxycarbonyl-Gln-p-nitrophenyl ester isoenzyme I and isoenzyme II Sus scrofa
0.13
-
 benzoyl-Phe-Val-Arg-p-nitroanilide isoenzyme II Sus scrofa
0.13
-
 benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-beta-naphthylamide isoenzyme I Sus scrofa
0.2
-
 benzyloxycarbonyl-Val-Lys-Lys-Arg-4-methoxy-beta-naphthylamide isoenzyme I Sus scrofa
0.43
-
 benzoyl-Pro-Phe-Arg-p-nitroanilide isoenzyme I Sus scrofa
0.8
-
 benzoyl-DL-Arg-beta-naphthylamide isoenzyme II Sus scrofa
0.91
-
 benzyloxycarbonyl-Val-Lys-Lys-Arg-4-methoxy-beta-naphthylamide isoenzyme II Sus scrofa
1
-
 benzoyl-Pro-Phe-Arg-p-nitroanilide isoenzyme II Sus scrofa
2.9
-
 benzoyl-DL-Arg-beta-naphthylamide isoenzyme I Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 isoenzyme I, 1 * 5000 + 1 * 23000, gel filtration, SDS-PAGE Sus scrofa
27000
-
 isoenzyme II, 1 * 27000, gel filtration, SDS-PAGE Sus scrofa
27000 33000 isoenzyme II, gel filtration, SDS-PAGE Sus scrofa
28000
-
 isoenzyme I Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Proteins + H2O Sus scrofa degradation of tissue proteins within lysosomes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-DL-Arg-beta-naphthylamide + H2O
-
 Sus scrofa benzoyl-DL-Arg + beta-naphthylamine
-
 ?
benzoyl-Phe-Val-Arg-p-nitroanilide + H2O
-
 Sus scrofa benzoyl-Phe-Val-Arg + p-nitroaniline
-
 ?
benzoyl-Pro-Phe-Arg-p-nitroanilide + H2O
-
 Sus scrofa benzoyl-Pro-Phe-Arg + p-nitroaniline
-
 ?
benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-beta-naphthylamide + H2O
-
 Sus scrofa benzyloxycarbonyl-Ala-Arg-Arg + 4-methoxy-beta-naphthylamine
-
 ?
benzyloxycarbonyl-Val-Lys-Lys-Arg-4-methoxy-beta-naphthylamide + H2O
-
 Sus scrofa ? + 4-methoxy-beta-naphthylamine
-
 ?
Glucagon + H2O
-
 Sus scrofa Hydrolyzed glucagon
-
 ?
N-t-butyloxycarbonyl-Gln-p-nitrophenyl ester + H2O
-
 Sus scrofa N-t-butyloxycarbonyl-Gln + 4-nitrophenol
-
 ?
Proteins + H2O degradation of tissue proteins within lysosomes Sus scrofa ?
-
 ?

Subunits

Subunits Comment Organism
dimer isoenzyme I, 1 * 5000 + 1 * 23000, gel filtration, SDS-PAGE Sus scrofa
monomer isoenzyme II, 1 * 27000, gel filtration, SDS-PAGE Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1
-
-
 Sus scrofa