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Literature summary for 3.4.22.1 extracted from

  • Wada, K.; Tanabe, T.
    Purification and characterization of chicken liver cathepsin B (1988), J. Biochem., 104, 472-476.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
antipain 93% inhibition at 1 microM Gallus gallus
chymostatin 85% inhibition at 5 microM Gallus gallus
iodoacetic acid 100% inhibition at 0.5 mM Gallus gallus
leupeptin 98% inhibition at 1 microM Gallus gallus
phenylmethanesulfonyl fluoride no inhibition at 0.1 mM Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide
-
Gallus gallus
20
-
benzoyl-DL-Arg-p-nitroanilide
-
Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24500
-
1 * 24500 or 25500 + 1 * 5000, SDS-PAGE Gallus gallus
28000
-
gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Proteins + H2O Gallus gallus turnover of intracellular proteins ?
-
?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-

Storage Stability

Storage Stability Organism
-80°C, pH 7.5, 3 months, stable Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-DL-Arg-p-nitroanilide + H2O
-
Gallus gallus benzoyl-DL-Arg + p-nitroaniline
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide
-
Gallus gallus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
?
Proteins + H2O turnover of intracellular proteins Gallus gallus ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 24500 or 25500 + 1 * 5000, SDS-PAGE Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide
-
Gallus gallus
83
-
benzoyl-DL-Arg-p-nitroanilide
-
Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7
-
Gallus gallus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 7.5 stable Gallus gallus