Any feedback?
Literature summary for 3.4.22.1 extracted from
- A comparison of four cathepsins (B, L, N and S) with collagenolytic activity from rabbit spleen (1988), Biochem. J., 256, 433-440.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | activates | Oryctolagus cuniculus |  |
| thiol compounds | - |
Oryctolagus cuniculus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cystatin | poor inhibitor, 51% inhibition at 100 nM | Oryctolagus cuniculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25250 | - |
gel filtration | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Collagen + H2O | Oryctolagus cuniculus | - |
? | - |
? | |
| Proteins + H2O | Oryctolagus cuniculus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spleen | - |
Oryctolagus cuniculus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, sodium acetate buffer, pH 5.0, several months, stable | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Oryctolagus cuniculus | fragments of azocasein | - |
? | |
| benzoyl-Phe-Val-Arg-4-methylcoumarin-7-amide + H2O | - |
Oryctolagus cuniculus | benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O | - |
Oryctolagus cuniculus | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide | - |
Oryctolagus cuniculus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| Collagen + H2O | - |
Oryctolagus cuniculus | Hydrolyzed collagen | - |
? | |
| Collagen + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| gelatin + H2O | - |
Oryctolagus cuniculus | hydrolyzed gelatine | - |
? | |
| Proteins + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000000001 | - |
azocasein | - |
Oryctolagus cuniculus | |
| 0.00000000817 | - |
gelatin | - |
Oryctolagus cuniculus | |
| 0.000625 | - |
Collagen | - |
Oryctolagus cuniculus | |
| 3.45 | - |
benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide | - |
Oryctolagus cuniculus | |
| 7.67 | - |
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide | - |
Oryctolagus cuniculus | |
| 8.12 | - |
benzoyl-Phe-Val-Arg-4-methylcoumaryl-7-amide | - |
Oryctolagus cuniculus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
