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Literature summary for 3.4.21.26 extracted from
- Proline-specific endopeptidase from Flavobacterium. Purification and properties (1980), J. Biol. Chem., 255, 4786-4792.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Chloromethylketone derivatives | - |
Elizabethkingia meningoseptica |  |
| diisopropylfluorophosphate | - |
Elizabethkingia meningoseptica | |
| additional information | - |
Elizabethkingia meningoseptica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Elizabethkingia meningoseptica | |
| 0.125 | - |
N-benzyloxycarbonyl-Gly-Pro-p-nitrophenyl ester | - |
Elizabethkingia meningoseptica | |
| 0.16 | - |
N-benzyloxycarbonyl-Ala-Ala-p-nitrophenyl ester | - |
Elizabethkingia meningoseptica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | 74000 | gel filtration | Elizabethkingia meningoseptica |
| 76000 | - |
? * 76000, SDS-PAGE | Elizabethkingia meningoseptica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Elizabethkingia meningoseptica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Elizabethkingia meningoseptica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Elizabethkingia meningoseptica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | cleavage of Pro-X bond | Elizabethkingia meningoseptica | ? | - |
? | |
| additional information | and more slowly Ala-X bond | Elizabethkingia meningoseptica | ? | - |
? | |
| additional information | not: (7-D-Pro)oxytocin and high molecular weight proteins even after denaturation | Elizabethkingia meningoseptica | ? | - |
? | |
| N-benzyloxycarbonyl-Ala-Ala-p-nitrophenyl ester + H2O | - |
Elizabethkingia meningoseptica | ? | - |
? | |
| N-benzyloxycarbonyl-Gly-Pro-p-nitrophenyl ester + H2O | - |
Elizabethkingia meningoseptica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | ? * 76000, SDS-PAGE | Elizabethkingia meningoseptica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Elizabethkingia meningoseptica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
15 min, 50% loss of activity | Elizabethkingia meningoseptica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Elizabethkingia meningoseptica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | 2 h | Elizabethkingia meningoseptica |
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Release 2023.1 (January 2023)
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