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Literature summary for 3.4.21.26 extracted from

  • Eugster, P.J.; Salamin, K.; Grouzmann, E.; Monod, M.
    Production and characterization of two major Aspergillus oryzae secreted prolyl endopeptidases able to efficiently digest proline-rich peptides of gliadin (2015), Microbiology, 161, 2277-2288 .
    View publication on PubMed
Search for more literature for 3.4.21.26

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen + H2O Aspergillus oryzae
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 7 glycosylation sites Aspergillus oryzae

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:soy meal protein-grown cell
-
 Aspergillus oryzae
-
culture condition:wheat gliadin-grown cell
-
 Aspergillus oryzae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Collagen + H2O
-
 Aspergillus oryzae ?
-
 ?
Gliadin + H2O digestion of the gliadin peptide in short peptides with both enzymes S28A and S28B, occur from its N terminus Aspergillus oryzae ?
-
 ?
L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae L-Ala-L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?
L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?
L-Ala-L-Pro-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae L-Ala-L-Pro-L-Pro + 4-nitroaniline
-
 ?
LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF + H2O
-
 Aspergillus oryzae ?
-
 ?
additional information no activity with L-Ala-L-Pro-4-nitroanilide and L-Ala-L-Pro-L-Phe-4-nitroanilide Aspergillus oryzae ?
-
 ?
succinyl-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae succinyl-L-Ala-L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 55528, isoform S28A, calculated from amino acid sequence Aspergillus oryzae
? x * 60000-100000, isoform S28B, SDS-PAGE Aspergillus oryzae
? x * 60000-70000, isoform S28A, SDS-PAGE Aspergillus oryzae
? x * 60242, isoform S28B, calculated from amino acid sequence Aspergillus oryzae

Synonyms

Synonyms Comment Organism
prolyl endopeptidase
-
 Aspergillus oryzae
S28A
-
 Aspergillus oryzae
S28B
-
 Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 isoform S28A Aspergillus oryzae
4.5
-
 isoform S28B Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
2 6 isoform S28A Aspergillus oryzae
2 7 isoform S28B Aspergillus oryzae

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus oryzae isoform S28A, calculated from amino acid sequence
-
 4.6
Aspergillus oryzae isoform S28B, calculated from amino acid sequence
-
 5.4