Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Galerina marginata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.051 | - |
IWGIGCNPWTAEHVDQTLASGNDIC | at pH 8.0 and 37°C | Galerina marginata | |
0.0554 | - |
IWGIGCNPWTAEHVDQTLASGNDIC | at pH 8.0 and 20°C | Galerina marginata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Galerina marginata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography, HiTrap Q column chromatography, and Superdex S200 gel filtration | Galerina marginata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
IWGIGCNPWTAEHVDQTLASGNDIC + H2O | a peptide with 25 amino acids (25mer, sequence IWGIGCNPWTAEHVDQTLASGNDIC) is utilized by the enzyme as a substrate for the macrocyclization reaction. During the macrocyclase reaction, the enzyme generates an eight-amino acid cyclic peptide from the N-terminal residues (the core, sequence IWGIGCNP), cleaving off the 17-C-terminal amino acid recognition sequence (peptide tail, sequence WTAEHVDQTLASGNDIC) | Galerina marginata | cyclic IWGIGCNP + WTAEHVDQTLASGNDIC | - |
? |
Synonyms | Comment | Organism |
---|---|---|
POPB | - |
Galerina marginata |
prolyl oligopeptidase B | - |
Galerina marginata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
IWGIGCNPWTAEHVDQTLASGNDIC | at pH 8.0 and 20°C | Galerina marginata | |
0.58 | - |
IWGIGCNPWTAEHVDQTLASGNDIC | at pH 8.0 and 37°C | Galerina marginata |