Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.26 extracted from

  • Kang, C.; Yu, X.W.; Xu, Y.
    Purification and characterization of a prolyl endopeptidase isolated from Aspergillus oryzae (2014), J. Ind. Microbiol. Biotechnol., 41, 49-55.
    View publication on PubMed
Search for more literature for 3.4.21.26

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 65% residual activity at 10 mM Aspergillus oryzae
Aprotinin 89% residual activity at 1 mM Aspergillus oryzae
Cu2+ 12% residual activity at 1 mM Aspergillus oryzae
EDTA 37.3% residual activity at 10 mM Aspergillus oryzae
Fe2+ 55% residual activity at 10 mM Aspergillus oryzae
leupeptin 86% residual activity at 1 mM Aspergillus oryzae
Mg2+ 97% residual activity at 10 mM Aspergillus oryzae
Mn2+ 30% residual activity at 10 mM Aspergillus oryzae
phenylmethylsulfonyl fluoride 97% residual activity at 1 mM Aspergillus oryzae
Zn2+ 91% residual activity at 10 mM Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 L-Ala-L-Ala-L-Pro-4-nitroanilide at pH 5.0 and 35°C Aspergillus oryzae
0.26
-
 Z-Gly-L-Pro-4-nitroanilide at pH 5.0 and 35°C Aspergillus oryzae
0.37
-
 Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide at pH 5.0 and 35°C Aspergillus oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 100% activity at 10 mM Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
casein + H2O Aspergillus oryzae
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, HiTrap DEAE column chromatography, and HiTrap Q column chromatography Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
 crude extract, at pH 5.0 and 35°C Aspergillus oryzae
3650
-
 after 73fold purification, at pH 5.0 and 35°C Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Aspergillus oryzae ?
-
 ?
L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?
Z-Gly-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae Z-Gly-L-Pro + 4-nitroaniline
-
 ?
Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Aspergillus oryzae Z-L-Ala-L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
POP
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 the residual activity after 30 min incubation at 55°C is higher than 80% Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
75
-
 Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide at pH 5.0 and 35°C Aspergillus oryzae
107.4
-
 L-Ala-L-Ala-L-Pro-4-nitroanilide at pH 5.0 and 35°C Aspergillus oryzae
240
-
 Z-Gly-L-Pro-4-nitroanilide at pH 5.0 and 35°C Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 5 more than 70% of the initial activity remains after incubation of the enzyme for 30 min at 35°C between pH 3.0 and 5.0 Aspergillus oryzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
24
-
 Z-Gly-L-Pro-4-nitroanilide at pH 5.0 and 35°C Aspergillus oryzae
202.7
-
 Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide at pH 5.0 and 35°C Aspergillus oryzae
596.7
-
 L-Ala-L-Ala-L-Pro-4-nitroanilide at pH 5.0 and 35°C Aspergillus oryzae