Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenylmethylsulfonyl fluoride | - |
Eurygaster integriceps |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06528 | - |
benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide | in 25 mM ethanolamine, pH 8.5, at 20°C | Eurygaster integriceps |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
x * 97000, SDS-PAGE | Eurygaster integriceps |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
gluten + H2O | Eurygaster integriceps | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eurygaster integriceps | - |
sunn pest | - |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, Cu2+ Sepharose column chromatography, and Sephacryl S-100 gel filtration | Eurygaster integriceps |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
crude extract and purified enzyme with gluten as substrate show specific activities of 1.0 and 4.5 units/mg protein, respectively, at pH 8.5 and 20°C (one unit is defined as change in gel height of 1 mm/h) | Eurygaster integriceps |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide + H2O | - |
Eurygaster integriceps | benzyloxycarbonyl-Gly-L-Pro + 4-nitroaniline | - |
? | |
gluten + H2O | - |
Eurygaster integriceps | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 97000, SDS-PAGE | Eurygaster integriceps |
Synonyms | Comment | Organism |
---|---|---|
glutenase | - |
Eurygaster integriceps |
prolyl endoprotease | - |
Eurygaster integriceps |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | 34 | - |
Eurygaster integriceps |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | 50 | approximately, 50% of the enzyme activity is retained at 38°C during the 20 min period of the assay. The enzyme also retains about 30% of its activity at 40°C but loses its activity entirely at 50°C | Eurygaster integriceps |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | between 20°C and 35°C, above pH 10, the activity declines sharply | Eurygaster integriceps |