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Literature summary for 3.4.21.26 extracted from

  • Bastos, I.M.; Motta, F.N.; Charneau, S.; Santana, J.M.; Dubost, L.; Augustyns, K.; Grellier, P.
    Prolyl oligopeptidase of Trypanosoma brucei hydrolyzes native collagen, peptide hormones and is active in the plasma of infected mice (2010), Microbes Infect., 12, 457-66.
    View publication on PubMed
Search for more literature for 3.4.21.26

Activating Compound

Activating Compound Comment Organism Structure
DTT in presence of DTT about 6fold higher catalytic efficiency of the enzyme Trypanosoma brucei brucei

Application

Application Comment Organism
medicine involvement in pathogenesis of sleeping sickness suggested Trypanosoma brucei brucei

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trypanosoma brucei brucei

Inhibitors

Inhibitors Comment Organism Structure
(3S)-N-benzyl-3-[[(2S)-2-(chloroacetyl)pyrrolidin-1-yl]carbonyl]-3,4-dihydroisoquinoline-2(1H)-carboxamide
-
 Trypanosoma brucei brucei
AEBSF 1 mM, 12% inhibition Trypanosoma brucei brucei
benzamidine 1 mM, 7.2% inhibition Trypanosoma brucei brucei
benzyl (2S)-2-([(2S)-2-[(5-phenyl-4,5-dihydro-1,2-oxazol-3-yl)carbonyl]pyrrolidin-1-yl]carbonyl)pyrrolidine-1-carboxylate
-
 Trypanosoma brucei brucei
benzyl (2S)-2-[[(2S)-2-([5-[(benzyloxy)methyl]-1,2-oxazol-3-yl]carbonyl)pyrrolidin-1-yl]carbonyl]pyrrolidine-1-carboxylate
-
 Trypanosoma brucei brucei
benzyl (2S)-2-[[(2S)-2-([5-[(benzyloxy)methyl]-4,5-dihydro-1,2-oxazol-3-yl]carbonyl)pyrrolidin-1-yl]carbonyl]pyrrolidine-1-carboxylate
-
 Trypanosoma brucei brucei
DFP 0.1 mM, 100% inhibition Trypanosoma brucei brucei
leupeptin 0.1 mM, 46.2% inhibition Trypanosoma brucei brucei
PCMB 0.1 mM, 98% inhibition Trypanosoma brucei brucei
PMSF 1 mM, 18% inhibition Trypanosoma brucei brucei
TLCK 0.1 mM, 73.2% inhibition Trypanosoma brucei brucei
TPCK 0.1 mM, 91.1% inhibition Trypanosoma brucei brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01521
-
 N-Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin in absence of DTT Trypanosoma brucei brucei
0.04413
-
 N-Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin in presence of DTT Trypanosoma brucei brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77600
-
 SDS-PAGE Trypanosoma brucei brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei Q6HA27
-
-

Source Tissue

Source Tissue Comment Organism Textmining
procyclic form
-
 Trypanosoma brucei brucei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-endorphin + h2O
-
 Trypanosoma brucei brucei Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro + Leu-Val-Thr-Leu-Phe-Lys-Asn-Ala + Ile-Ile-Lys-Asn-Ala + Tyr-Lys-Lys-Gly-Glu
-
 ?
bradykinin + H2O
-
 Trypanosoma brucei brucei Arg-Pro-Pro + Gly-Phe-Ser-Pro + Phe-Arg
-
 ?
collagen I + H2O assay at pH 8.0, 37°C Trypanosoma brucei brucei ?
-
 ?
GnRH + H2O
-
 Trypanosoma brucei brucei pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro + Gly-NH2
-
 ?
N-Suc-Ala-Ala-Ala-7-amido-4-methylcoumarin + H2O assay at pH 7.5, 25°C Trypanosoma brucei brucei N-Suc-Ala-Ala-Ala + 7-amino-4-methylcoumarin
-
 ?
N-Suc-Gly-Pro-7-amido-4-methylcoumarin + H2O assay at pH 7.5, 25°C Trypanosoma brucei brucei N-Suc-Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
N-Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin + H2O assay at pH 7.5, 25°C Trypanosoma brucei brucei N-Suc-Gly-Pro-Leu-Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
neurotensin + H2O
-
 Trypanosoma brucei brucei pGlu-Leu-Tyr-Glu-Asn-Lys-Pro + Arg-Arg-Pro + Tyr-Ile-Leu
-
 ?
TRH + H2O
-
 Trypanosoma brucei brucei L-pyroglutamyl-L-histidyl-L-proline + NH3
-
 ?

Synonyms

Synonyms Comment Organism
POP Tb
-
 Trypanosoma brucei brucei
prolyl oligopeptidase
-
 Trypanosoma brucei brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Trypanosoma brucei brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Trypanosoma brucei brucei

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8
-
 Trypanosoma brucei brucei

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 16% enzyme activity at pH 6.0, 62% enzyme activity at pH 9.0 Trypanosoma brucei brucei

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00000034
-
-
 Trypanosoma brucei brucei benzyl (2S)-2-([(2S)-2-[(5-phenyl-4,5-dihydro-1,2-oxazol-3-yl)carbonyl]pyrrolidin-1-yl]carbonyl)pyrrolidine-1-carboxylate
0.0000014
-
-
 Trypanosoma brucei brucei benzyl (2S)-2-[[(2S)-2-([5-[(benzyloxy)methyl]-4,5-dihydro-1,2-oxazol-3-yl]carbonyl)pyrrolidin-1-yl]carbonyl]pyrrolidine-1-carboxylate
0.000002
-
-
 Trypanosoma brucei brucei (3S)-N-benzyl-3-[[(2S)-2-(chloroacetyl)pyrrolidin-1-yl]carbonyl]-3,4-dihydroisoquinoline-2(1H)-carboxamide
0.0000021
-
-
 Trypanosoma brucei brucei benzyl (2S)-2-[[(2S)-2-([5-[(benzyloxy)methyl]-1,2-oxazol-3-yl]carbonyl)pyrrolidin-1-yl]carbonyl]pyrrolidine-1-carboxylate

General Information

General Information Comment Organism
physiological function important role in host interactions Trypanosoma brucei brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
33
-
 N-Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin in absence of DTT Trypanosoma brucei brucei
209
-
 N-Suc-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin in presence of DTT Trypanosoma brucei brucei