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Literature summary for 3.4.21.26 extracted from

  • Nolte, W.M.; Tagore, D.M.; Lane, W.S.; Saghatelian, A.
    Peptidomics of prolyl endopeptidase in the central nervous system (2009), Biochemistry, 48, 11971-11981.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Rosetta2 cells and in COS-7 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
D35A/K196A reduced specificity for shorter peptides Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
calcitonin gene-related peptide
-
Mus musculus
S17092
-
Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
calcitonin gene-related peptide mutant D35A/K196A, shorter peptide of calcitonin gene-related peptide Mus musculus
0.0034
-
calcitonin gene-related peptide mutant D35A/K196A, diserine mutant of calcitonin gene-related peptide Mus musculus
0.0043
-
calcitonin gene-related peptide wild type enzyme, full length calcitonin gene-related peptide Mus musculus
0.01
-
calcitonin gene-related peptide wild type enzyme, shorter peptide of calcitonin gene-related peptide Mus musculus
0.0125
-
calcitonin gene-related peptide wild type enzyme, diserine mutant of calcitonin gene-related peptide Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
calcitonin gene-related peptide + H2O assay at pH 7.0, 37°C Mus musculus ?
-
?
Substance P + H2O
-
Mus musculus ?
-
?
Z-Gly-Pro-7-amido-4-methylcoumarin + H2O assay at pH 7.0, 37°C Mus musculus z-Gly-Pro + 7-amino-4-methylcoumarin
-
?

Synonyms

Synonyms Comment Organism
PREP
-
Mus musculus
prolyl endopeptidase
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0012
-
calcitonin gene-related peptide wild type enzyme, full length calcitonin gene-related peptide Mus musculus
0.002
-
calcitonin gene-related peptide wild type enzyme, diserine mutant of calcitonin gene-related peptide Mus musculus
0.0023
-
calcitonin gene-related peptide mutant D35A/K196A, diserine mutant of calcitonin gene-related peptide Mus musculus
0.0051
-
calcitonin gene-related peptide mutant D35A/K196A, shorter peptide of calcitonin gene-related peptide Mus musculus
0.072
-
calcitonin gene-related peptide wild type enzyme, shorter peptide of calcitonin gene-related peptide Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.002
-
-
Mus musculus calcitonin gene-related peptide

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.16
-
calcitonin gene-related peptide wild type enzyme, diserine mutant of calcitonin gene-related peptide Mus musculus
0.22
-
calcitonin gene-related peptide mutant D35A/K196A, full length calcitonin gene-related peptide Mus musculus
0.28
-
calcitonin gene-related peptide wild type enzyme, full length calcitonin gene-related peptide Mus musculus
0.66
-
calcitonin gene-related peptide mutant D35A/K196A, diserine mutant of calcitonin gene-related peptide Mus musculus
4.3
-
calcitonin gene-related peptide mutant D35A/K196A, shorter peptide of calcitonin gene-related peptide Mus musculus
6.9
-
calcitonin gene-related peptide wild type enzyme, shorter peptide of calcitonin gene-related peptide Mus musculus