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Literature summary for 3.4.21.26 extracted from

  • Tenorio-Laranga, J.; Venaelaeinen, J.I.; Maennistoe, P.T.; Garcia-Horsman, J.A.
    Characterization of membrane-bound prolyl endopeptidase from brain (2008), FEBS J., 275, 4415-4427.
    View publication on PubMed
Search for more literature for 3.4.21.26

Inhibitors

Inhibitors Comment Organism Structure
alpha2-gliadin 33-mer peptide POP inhibitor Sus scrofa
JTP-4819
-
 Sus scrofa
additional information specific dipeptidyl peptidase IV inhibitor HIV-1 tat (1-9) fragment with sequence H-Met-Asp-Pro-Val-Asp-Pro-Asn-Ile-Glu-OH Sus scrofa
N-carbobenzoxy-Leu-Leu-Leu-COH the proteasome inhibitor Sus scrofa
N-carbobenzoxy-prolyl-prolinal ZPP Sus scrofa
phenylmethanesulfonyl fluoride PE and POP are partially resistant to phenylmethanesulfonyl fluoride, a generic serine protease inhibitor that has a low efficiency in inhibiting POP Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Sus scrofa 5737
-
extracellular
-
 Sus scrofa
-
-
membrane
-
 Sus scrofa 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+
-
 Sus scrofa
Mn2+
-
 Sus scrofa
additional information no significant differences between membrane PE and POP regarding the sensitivity to the metals Sus scrofa
NaCl
-
 Sus scrofa
Ni2+
-
 Sus scrofa
Zn2+
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 higher salt concentrations used, membrane PE activity elutes with a molecular mass of 65 kDa Sus scrofa
80000
-
 theoretical Sus scrofa
95000
-
 membrane PE, conditions: 20 mm potassium phosphate, gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00017
-
 high-density membranes, membrane-bound POP activity of different-density membrane fractions obtained by sucrose gradient centrifugation Sus scrofa
0.0002
-
 washed membranes, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity Sus scrofa
0.00045
-
 medium-density membranes, membrane-bound POP activity of different-density membrane fractions obtained by sucrose gradient centrifugation Sus scrofa
0.0005
-
 total membranes, purification of mPOP from a total membrane preparation Sus scrofa
0.0005
-
 washed membranes, membrane-bound POP activity of different-density membrane fractions obtained by sucrose gradient centrifugation Sus scrofa
0.00061
-
 low-density membranes, membrane-bound POP activity of different-density membrane fractions obtained by sucrose gradient centrifugation Sus scrofa
0.001
-
 crude extract, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity Sus scrofa
0.0014
-
 unwashed membranes, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity Sus scrofa
0.0033
-
 Triton X-100 extraction, purification of mPOP from a total membrane preparation Sus scrofa
0.0048
-
 4 M NaCl wash, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity Sus scrofa
0.0074
-
 DEAE, purification of mPOP from a total membrane preparation Sus scrofa
0.009
-
 0.5 M NaCl wash, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity Sus scrofa
0.0138
-
 water wash, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity Sus scrofa
0.039
-
 phenyl-sepharose, purification of mPOP from a total membrane preparation Sus scrofa
0.169
-
 hydroxylapatite, purification of mPOP from a total membrane preparation Sus scrofa
0.857
-
 second DEAE, purification of mPOP from a total membrane preparation Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-CDPGYIGSR-NH2 + H2O substrate specificity studies on membrane PE as compared with POP. ZPP-sensitive cleavage of both occurred Sus scrofa ?
-
 ?
H-MDPVDPNIE-OH + H2O substrate specificity studies on membrane PE as compared with POP. ZPP-sensitive cleavage of both occurred Sus scrofa ?
-
 ?
H2N-QLQPFPQPQLPY-OH + H2O substrate specificity studies on membrane PE as compared with POP. ZPP-sensitive cleavage of both occurred Sus scrofa ?
-
 ?
additional information substance P, arginine-vasopressin, thyroliberin and gonadoliberin are proposed physiological substrates of this protease. No ZPP-sensitive cleavage occurred with the 33-mer, PEP-3, PEP-26, PEP-48_2, and PEP-50 Sus scrofa ?
-
 ?
N-carbobenzoxy-Gly-Pro-7-amido-4-methyl-coumarin + H2O
-
 Sus scrofa ?
-
 ?

Synonyms

Synonyms Comment Organism
membrane-bound PE
-
 Sus scrofa
mPOP
-
 Sus scrofa
POP
-
 Sus scrofa
prolyl endopeptidase
-
 Sus scrofa