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Literature summary for 3.4.21.26 extracted from

  • Gorrao, S.S.; Hemerly, J.P.; Lima, A.R.; Melo, R.L.; Szeltner, Z.; Polgar, L.; Juliano, M.A.; Juliano, L.
    Fluorescence resonance energy transfer (FRET) peptides and cycloretro-inverso peptides derived from bradykinin as substrates and inhibitors of prolyl oligopeptidase (2007), Peptides, 28, 2146-2154.
    View publication on PubMed
Search for more literature for 3.4.21.26

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain JM105 Sus scrofa

Protein Variants

Protein Variants Comment Organism
C255T site-directed mutagenesis, mutant substrate specificity and kinetics in comparison to the wild-type enzyme Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Z-Pro-Pro-dimethyl acetal aldehyde irreversible inhibitor Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics and substrate specificity, overview Sus scrofa
0.00024
-
 2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
0.00024
-
 2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
0.00033
-
 2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
0.00036
-
 2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant mutant C255T Sus scrofa
0.00042
-
 2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
0.00047
-
 2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant mutant C255T Sus scrofa
0.00082
-
 2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant mutant C255T Sus scrofa
0.00084
-
 2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Ile-Gly-GLu-Ile-Lys-Glu-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
0.0009
-
 2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
0.00111
-
 2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant mutant C255T Sus scrofa
0.00117
-
 2-aminobenzoyl-Gly-Pro-Phe-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
0.00141
-
 2-aminobenzoyl-Gly-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
0.00154
-
 2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Ile-Gly-GLu-Ile-Lys-Glu-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant mutant C255T Sus scrofa
0.0019
-
 2-aminobenzoyl-Gly-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
0.00219
-
 2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol active enzyme form Sus scrofa 5829
-

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O the fluorescence resonance energy transfer peptide sequence corresponds to bradykinin from human kininogen Sus scrofa 2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro + Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O the fluorescence resonance energy transfer peptide sequence corresponds to bradykinin from human kininogen Sus scrofa 2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro + Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O a fluorescence resonance energy transfer peptide Sus scrofa 2-aminobenzoyl-Gly-Phe-Ser-Pro + Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O the fluorescence resonance energy transfer peptide sequence corresponds to bradykinin from human kininogen Sus scrofa 2-aminobenzoyl-Gly-Phe-Ser-Pro + Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Ile-Gly-GLu-Ile-Lys-Glu-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O the fluorescence resonance energy transfer peptide sequence corresponds to bradykinin from human kininogen Sus scrofa 2-aminobenzoyl-Gly-Phe-Ser-Pro + Phe-Arg-Ser-Ser-Arg-Ile-Gly-GLu-Ile-Lys-Glu-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O a fluorescence resonance energy transfer peptide Sus scrofa 2-aminobenzoyl-Gly-Pro + Gln-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O the fluorescence resonance energy transfer peptide sequence corresponds to bradykinin from human kininogen Sus scrofa 2-aminobenzoyl-Gly-Pro + Gln-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
2-aminobenzoyl-Gly-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O a fluorescence resonance energy transfer peptide Sus scrofa 2-aminobenzoyl-Gly-Pro + Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
2-aminobenzoyl-Gly-Pro-Phe-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O a fluorescence resonance energy transfer peptide Sus scrofa 2-aminobenzoyl-Gly-Pro + Phe-Gln-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
2-aminobenzoyl-Gly-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O a fluorescence resonance energy transfer peptide Sus scrofa 2-aminobenzoyl-Gly-Ser-Pro + Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
additional information substrate specificity, overview Sus scrofa ?
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme is a member of the serine peptidase family with post-proline cleaving activity towards peptides Sus scrofa
POP
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant mutant C255T Sus scrofa
0.6
-
 2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Ile-Gly-GLu-Ile-Lys-Glu-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme and mutant C255T Sus scrofa
0.9
-
 2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
1
-
 2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant mutant C255T Sus scrofa
1.1
-
 2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
1.4
-
 2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
1.4
-
 2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
1.4
-
 2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant mutant C255T Sus scrofa
1.5
-
 2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
1.8
-
 2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant mutant C255T Sus scrofa
3.5
-
 2-aminobenzoyl-Gly-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
4
-
 2-aminobenzoyl-Gly-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
6
-
 2-aminobenzoyl-Gly-Pro-Phe-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa
7.9
-
 2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine pH 7.5, 37°C, recombinant wild-type enzyme Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.5 assay at Sus scrofa