Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain JM105 | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
C255T | site-directed mutagenesis, mutant substrate specificity and kinetics in comparison to the wild-type enzyme | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Z-Pro-Pro-dimethyl acetal aldehyde | irreversible inhibitor | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and substrate specificity, overview | Sus scrofa | |
0.00024 | - |
2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
0.00024 | - |
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
0.00033 | - |
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
0.00036 | - |
2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant mutant C255T | Sus scrofa | |
0.00042 | - |
2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
0.00047 | - |
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant mutant C255T | Sus scrofa | |
0.00082 | - |
2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant mutant C255T | Sus scrofa | |
0.00084 | - |
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Ile-Gly-GLu-Ile-Lys-Glu-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
0.0009 | - |
2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
0.00111 | - |
2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant mutant C255T | Sus scrofa | |
0.00117 | - |
2-aminobenzoyl-Gly-Pro-Phe-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
0.00141 | - |
2-aminobenzoyl-Gly-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
0.00154 | - |
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Ile-Gly-GLu-Ile-Lys-Glu-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant mutant C255T | Sus scrofa | |
0.0019 | - |
2-aminobenzoyl-Gly-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
0.00219 | - |
2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | active enzyme form | Sus scrofa | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O | the fluorescence resonance energy transfer peptide sequence corresponds to bradykinin from human kininogen | Sus scrofa | 2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro + Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | - |
? | |
2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O | the fluorescence resonance energy transfer peptide sequence corresponds to bradykinin from human kininogen | Sus scrofa | 2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro + Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | - |
? | |
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O | a fluorescence resonance energy transfer peptide | Sus scrofa | 2-aminobenzoyl-Gly-Phe-Ser-Pro + Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | - |
? | |
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O | the fluorescence resonance energy transfer peptide sequence corresponds to bradykinin from human kininogen | Sus scrofa | 2-aminobenzoyl-Gly-Phe-Ser-Pro + Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | - |
? | |
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Ile-Gly-GLu-Ile-Lys-Glu-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O | the fluorescence resonance energy transfer peptide sequence corresponds to bradykinin from human kininogen | Sus scrofa | 2-aminobenzoyl-Gly-Phe-Ser-Pro + Phe-Arg-Ser-Ser-Arg-Ile-Gly-GLu-Ile-Lys-Glu-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine | - |
? | |
2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O | a fluorescence resonance energy transfer peptide | Sus scrofa | 2-aminobenzoyl-Gly-Pro + Gln-N-(2,4-dinitrophenyl)ethylenediamine | - |
? | |
2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O | the fluorescence resonance energy transfer peptide sequence corresponds to bradykinin from human kininogen | Sus scrofa | 2-aminobenzoyl-Gly-Pro + Gln-N-(2,4-dinitrophenyl)ethylenediamine | - |
? | |
2-aminobenzoyl-Gly-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O | a fluorescence resonance energy transfer peptide | Sus scrofa | 2-aminobenzoyl-Gly-Pro + Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | - |
? | |
2-aminobenzoyl-Gly-Pro-Phe-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O | a fluorescence resonance energy transfer peptide | Sus scrofa | 2-aminobenzoyl-Gly-Pro + Phe-Gln-N-(2,4-dinitrophenyl)ethylenediamine | - |
? | |
2-aminobenzoyl-Gly-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O | a fluorescence resonance energy transfer peptide | Sus scrofa | 2-aminobenzoyl-Gly-Ser-Pro + Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | - |
? | |
additional information | substrate specificity, overview | Sus scrofa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a member of the serine peptidase family with post-proline cleaving activity towards peptides | Sus scrofa |
POP | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant mutant C255T | Sus scrofa | |
0.6 | - |
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Ile-Gly-GLu-Ile-Lys-Glu-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme and mutant C255T | Sus scrofa | |
0.9 | - |
2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
1 | - |
2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant mutant C255T | Sus scrofa | |
1.1 | - |
2-aminobenzoyl-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
1.4 | - |
2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
1.4 | - |
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
1.4 | - |
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant mutant C255T | Sus scrofa | |
1.5 | - |
2-aminobenzoyl-Gly-Phe-Ser-Pro-Phe-Arg-Ser-Ser-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
1.8 | - |
2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant mutant C255T | Sus scrofa | |
3.5 | - |
2-aminobenzoyl-Gly-Ser-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
4 | - |
2-aminobenzoyl-Gly-Pro-Phe-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
6 | - |
2-aminobenzoyl-Gly-Pro-Phe-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa | |
7.9 | - |
2-aminobenzoyl-Gly-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine | pH 7.5, 37°C, recombinant wild-type enzyme | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.5 | assay at | Sus scrofa |