Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and anaylsis, expression of tagged enzyme in Escherichia coli strains DH5alpha and BL21 | Thermococcus onnurineus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
N-succinyl-Ala-Pro-4-nitroanilide | pH 7.5, 80°C, recombinant enzyme | Thermococcus onnurineus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
recombinant enzyme, gel filtration | Thermococcus onnurineus |
70380 | - |
1 * 70380, sequence calculation, 1 * 72000, recombinant enzyme, SDS-PAGE | Thermococcus onnurineus |
72000 | - |
1 * 70380, sequence calculation, 1 * 72000, recombinant enzyme, SDS-PAGE | Thermococcus onnurineus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus onnurineus | Q2QC90 | isolated from the deep-sea hydrohermal vent area of the PACMANUS field in the East Manus Basin | - |
Purification (Comment) | Organism |
---|---|
recombinant tagged enzyme from Escherichia coli strain BL21 by metal affinity chromatography | Thermococcus onnurineus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Hydrolysis of --Pro-/- and to a lesser extent --Ala-/- in oligopeptides | catalytic residues are Ser477, Asp559, and His591 | Thermococcus onnurineus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-Ala-Pro-4-nitroanilide + H2O | - |
Thermococcus onnurineus | N-succinyl-Ala-Pro + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 70380, sequence calculation, 1 * 72000, recombinant enzyme, SDS-PAGE | Thermococcus onnurineus |
Synonyms | Comment | Organism |
---|---|---|
POP | - |
Thermococcus onnurineus |
TNA1_POP | - |
Thermococcus onnurineus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
temperatures of 80-90°C activate the enzyme | Thermococcus onnurineus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 100 | 25% of maximal activity within the range of 30-50°C, and at 100°C | Thermococcus onnurineus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
23 h, 80% remaining activity | Thermococcus onnurineus |
90 | - |
half-life is 3 h, loss of activity is due to autodegradation, not thermal inactivation, overview | Thermococcus onnurineus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22.2 | - |
N-succinyl-Ala-Pro-4-nitroanilide | pH 7.5, 80°C, recombinant enzyme | Thermococcus onnurineus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermococcus onnurineus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | - |
Thermococcus onnurineus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermococcus onnurineus | sequence calculaiton | - |
4.98 |