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Literature summary for 3.4.21.26 extracted from

  • Lee, H.S.; Kim, Y.J.; Cho, Y.; Kim, S.J.; Lee, J.H.; Kang, S.G.
    Characterization of prolyl oligopeptidase from hyperthermophilic archaeon Thermococcus sp. NA1 (2007), J. Biosci. Bioeng., 103, 221-228.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and anaylsis, expression of tagged enzyme in Escherichia coli strains DH5alpha and BL21 Thermococcus onnurineus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
N-succinyl-Ala-Pro-4-nitroanilide pH 7.5, 80°C, recombinant enzyme Thermococcus onnurineus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
recombinant enzyme, gel filtration Thermococcus onnurineus
70380
-
1 * 70380, sequence calculation, 1 * 72000, recombinant enzyme, SDS-PAGE Thermococcus onnurineus
72000
-
1 * 70380, sequence calculation, 1 * 72000, recombinant enzyme, SDS-PAGE Thermococcus onnurineus

Organism

Organism UniProt Comment Textmining
Thermococcus onnurineus Q2QC90 isolated from the deep-sea hydrohermal vent area of the PACMANUS field in the East Manus Basin
-

Purification (Commentary)

Purification (Comment) Organism
recombinant tagged enzyme from Escherichia coli strain BL21 by metal affinity chromatography Thermococcus onnurineus

Reaction

Reaction Comment Organism Reaction ID
Hydrolysis of --Pro-/- and to a lesser extent --Ala-/- in oligopeptides catalytic residues are Ser477, Asp559, and His591 Thermococcus onnurineus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-Ala-Pro-4-nitroanilide + H2O
-
Thermococcus onnurineus N-succinyl-Ala-Pro + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
monomer 1 * 70380, sequence calculation, 1 * 72000, recombinant enzyme, SDS-PAGE Thermococcus onnurineus

Synonyms

Synonyms Comment Organism
POP
-
Thermococcus onnurineus
TNA1_POP
-
Thermococcus onnurineus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
temperatures of 80-90°C activate the enzyme Thermococcus onnurineus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 100 25% of maximal activity within the range of 30-50°C, and at 100°C Thermococcus onnurineus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
23 h, 80% remaining activity Thermococcus onnurineus
90
-
half-life is 3 h, loss of activity is due to autodegradation, not thermal inactivation, overview Thermococcus onnurineus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22.2
-
N-succinyl-Ala-Pro-4-nitroanilide pH 7.5, 80°C, recombinant enzyme Thermococcus onnurineus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Thermococcus onnurineus

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
Thermococcus onnurineus

pI Value

Organism Comment pI Value Maximum pI Value
Thermococcus onnurineus sequence calculaiton
-
4.98