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Literature summary for 3.4.19.12 extracted from

  • Wang, D.H.; Song, W.; Wei, S.W.; Zheng, Y.F.; Chen, Z.S.; Han, J.D.; Zhang, H.T.; Luo, J.C.; Qin, Y.M.; Xu, Z.H.; Bai, S.N.
    Characterization of the ubiquitin C-terminal hydrolase and ubiquitin-specific protease families in rice (Oryza sativa) (2018), Front. Plant Sci., 9, 1636 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.19.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed by the pCold TF cold shock expression system in Escherichia coli Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000173
-
 NEDD8-7-amido-4-methylcoumarin pH 8.0, temperature not specified in the publication, ubiquitin C-terminal hydrolase OsUCH3 Oryza sativa
0.000185
-
 ubiquitin-7-amido-4-methylcoumarin pH 8.0, temperature not specified in the publication, ubiquitin C-terminal hydrolase OsUCH3 Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 ssp. japonica cv. Zhonghua11
-
Oryza sativa Q6ETK2 ssp. japonica cv. Zhonghua11
-
Oryza sativa Q6K6Z5 ssp. japonica cv. Zhonghua11
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
stamen
-
 Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NEDD8-7-amido-4-methylcoumarin + H2O
-
 Oryza sativa NEDD8 + 7-amino-4-methylcoumarin
-
 ?
ubiquitin-7-amido-4-methylcoumarin + H2O
-
 Oryza sativa ubiquitin + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
OsUCH1 Os02g08370 Oryza sativa
OsUCH2 Os02g57630 Oryza sativa
OsUCH3 Os02g43760 Oryza sativa
OsUCH4 Os04g46190 Oryza sativa
OsUCH5 Os04g57190 Oryza sativa
ubiquitin C-terminal hydrolase
-
 Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.46
-
 ubiquitin-7-amido-4-methylcoumarin pH 8.0, temperature not specified in the publication, ubiquitin C-terminal hydrolase OsUCH3 Oryza sativa
17.22
-
 NEDD8-7-amido-4-methylcoumarin pH 8.0, temperature not specified in the publication, ubiquitin C-terminal hydrolase OsUCH3 Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
40324
-
 ubiquitin-7-amido-4-methylcoumarin pH 8.0, temperature not specified in the publication, ubiquitin C-terminal hydrolase OsUCH3 Oryza sativa
99537
-
 NEDD8-7-amido-4-methylcoumarin pH 8.0, temperature not specified in the publication, ubiquitin C-terminal hydrolase OsUCH3 Oryza sativa