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Literature summary for 3.4.19.12 extracted from

  • Misaghi, S.; Ottosen, S.; Izrael-Tomasevic, A.; Arnott, D.; Lamkanfi, M.; Lee, J.; Liu, J.; ORourke, K.; Dixit, V.M.; Wilson, A.C.
    Association of C-terminal ubiquitin hydrolase BAP1 with cell cycle regulator HCF-1 (2009), Mol. Cell. Biol., 29, 2181-2192.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
Flag-tagged wild-type or mutant Bap1 expressed in HEK-293 cells. HA-tagged Bap1 transiently expressed in HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C91A catalytically inactive mutant, can still associate with the HCF-1 beta-propeller but cannot remove ubiquitin chains Homo sapiens
additional information wild type Bap1 and the NHNY/AAAA mutant display robust activity. Mutation of the NHNY sequence abolishes the association with HCF-1 but does not alter the intrinsic enzymatic activity of the ubiquitin C-terminal hydrolase domain Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
siRNA stabilization of HCF-1 through depletion of Bap1. Knockdown of Bap1 results in a modest increase in the steady-state levels of HCF-1, which presumably helps to promote the transition from G1 into S-phase Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm only Bap1, no presence of HCF-1 Homo sapiens 5737
-
nucleus HCF-1 and Bap1 are similarly distributed throughout the nucleoplasm. HCF-1 interacts with Bap1 through its beta-propeller domain Homo sapiens 5634
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens Bap1 helps to control cell proliferation by regulating HCF-1 protein levels and by associating with genes involved in the G1-S transition ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q92560
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-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant Bap1-flag proteins immunopurified with alpha-flag beads Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
HCF-1 + H2O HCF-1 is the principal Bap1-interacting protein. It is K48- and K63-ubiquitinated. Endogenous HCF-1C is ubiquitinated at lysine-1807 and/or lysine-1808 Homo sapiens ?
-
 ?
additional information Bap1 helps to control cell proliferation by regulating HCF-1 protein levels and by associating with genes involved in the G1-S transition Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
BAP1
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 Homo sapiens
BRCA1-associated protein-1
-
 Homo sapiens
C-terminal ubiquitin hydrolase
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 Homo sapiens