Activating Compound | Comment | Organism | Structure |
---|---|---|---|
TNFalpha | inducer | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression vectors encoding HA epitope-tagged Cezanne (pHM6-Cezanne), GFP-tagged Cezanne (pEGFP-Cezanne) and enzymatically inactive forms mutated at the catalytic cysteine (pHM6-Cezanne C209S and pEGFP-Cezanne C209S). Expressed in HEK293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C209S | catalytically inactive, does not suppress NF-kappaB nuclear translocation and transcriptional activity by targeting the TNFR signaling pathway at the level of the IkappaB kinase complex or upstream from it. Does not suppress polyubiquitinated RIP1 and does not enhance the levels of monubiquinated or unmodified RIP1 as compared to the wild-type | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HUVEC cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cezanne-specific siRNA suppresses Cezanne mRNA and protein by at least 70% in culture cells. It leads to elevated NF-kappaB luciferase reporter gene activity and enhanced expression of IL-8 transcripts in TNFalpha-treated cells. Endogenous Cezanne can attenuate NF-kappaB activation and the induction of pro-inflammatory transcripts in response to TNF receptor signaling. Deubiquitinating activity of Cezanne is essential for NF-kappB suppression. Cezanne can be recruited to activated TNF receptors where it suppresses the build-up of polyubiquitinated RIP1 signal adapter proteins | Homo sapiens | ? | - |
? | |
polyubiquitin + H2O | Cezanne deubiquitinates Lys48- or Lys63-polyubiquitinated cellular proteins | Homo sapiens | ubiquitin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
A20 | - |
Homo sapiens |
Cezanne | - |
Homo sapiens |