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Literature summary for 3.4.19.12 extracted from

  • Moon, W.J.; Hwang, D.K.; Park, E.J.; Kim, Y.M.; Chae, Y.K.
    Recombinant expression, isotope labeling, refolding, and purification of an antimicrobial peptide, piscidin (2007), Protein Expr. Purif., 51, 141-146.
    View publication on PubMed

Application

Application Comment Organism
additional information YUH effciently cleaves the ubiquitin-piscidin fusion protein, and since it can easily be prepared in the lab, it provides a cost-effective way of preparing the antimicrobial target peptide piscidin as compared to other systems Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
YUH gene ligated with the pET-23d vector, expressed in Escherichia coli Rosetta(DE3)pLysS Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P35127
-
-

Purification (Commentary)

Purification (Comment) Organism
by two purification steps, gel filtration Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, 20 mM sodium phosphate buffer, pH 7.4, 100% glycerol Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ubiquitin-piscidin 1 + H2O
-
Saccharomyces cerevisiae ubiquitin + piscidin 1
-
?

Synonyms

Synonyms Comment Organism
yeast ubiquitin hydrolase
-
Saccharomyces cerevisiae
YUH
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Saccharomyces cerevisiae