Application | Comment | Organism |
---|---|---|
additional information | YUH effciently cleaves the ubiquitin-piscidin fusion protein, and since it can easily be prepared in the lab, it provides a cost-effective way of preparing the antimicrobial target peptide piscidin as compared to other systems | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
YUH gene ligated with the pET-23d vector, expressed in Escherichia coli Rosetta(DE3)pLysS | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P35127 | - |
- |
Purification (Comment) | Organism |
---|---|
by two purification steps, gel filtration | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, 20 mM sodium phosphate buffer, pH 7.4, 100% glycerol | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquitin-piscidin 1 + H2O | - |
Saccharomyces cerevisiae | ubiquitin + piscidin 1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
yeast ubiquitin hydrolase | - |
Saccharomyces cerevisiae |
YUH | - |
Saccharomyces cerevisiae |