Literature summary for 3.4.19.12 extracted from

Yao, T.; Song, L.; Xu, W.; DeMartino, G.N.; Florens, L.; Swanson, S.K.; Washburn, M.P.; Conaway, R.C.; Conaway, J.W.; Cohen, R.E.
Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1 (2006), Nat. Cell Biol., 8, 994-1002.

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Activating Compound

Activating Compound	Comment	Organism	Structure
Adrm1	recruits Uch37 to the proteasome. Binds the carboxy-terminal tail of Uch37. Following binding, Adrm1 relieves Uch37 autoinhibition, accelerating the hydrolysis of ubiquitin-7-amido-4-methylcoumarin	Homo sapiens

Application

Application	Comment	Organism
additional information	Uch37 is responsible for isopeptidase activity	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
Uch37N (amino acids 1-237) expressed from pET21a with a C-terminal 6× histidine tag in Escherichia coli BL21(DE3). HEK293 cells stably expressing Uch37 containing an amino-terminal 2 × HA epitope. Uch37 expressed as a carboxy- or amino-terminal fusion to the Gal4 activation domain or the Gal4 DNA-binding domain. GST-Uch37, GST-Uch37N (1-237), GST-Uch37C (238-329), wild-type 6× His-Flag-Uch37 and mutant C88A subcloned into pBacPAK8 with their respective epitope tags and expressed using the BacPAK baculovirus expression system, expressed in Sf21 insect cells. DNA encoding human Uch37FL, Uch37N (1-237), Adrm1, and the 19S complex subunits S1, S2, S3, S4, S5a, S5b, S6, S6, S7, S8, S9, S10a, S10b, S11, S12, S13, S14, S15 and p55 subcloned into pGAD or pGBD yeast two-hybrid vectors and transformed into the YRG-2 yeast strain	Homo sapiens

Cloned (Comment)

Organism

Uch37N (amino acids 1-237) expressed from pET21a with a C-terminal 6× histidine tag in Escherichia coli BL21(DE3). HEK293 cells stably expressing Uch37 containing an amino-terminal 2 × HA epitope. Uch37 expressed as a carboxy- or amino-terminal fusion to the Gal4 activation domain or the Gal4 DNA-binding domain. GST-Uch37, GST-Uch37N (1-237), GST-Uch37C (238-329), wild-type 6× His-Flag-Uch37 and mutant C88A subcloned into pBacPAK8 with their respective epitope tags and expressed using the BacPAK baculovirus expression system, expressed in Sf21 insect cells. DNA encoding human Uch37FL, Uch37N (1-237), Adrm1, and the 19S complex subunits S1, S2, S3, S4, S5a, S5b, S6, S6, S7, S8, S9, S10a, S10b, S11, S12, S13, S14, S15 and p55 subcloned into pGAD or pGBD yeast two-hybrid vectors and transformed into the YRG-2 yeast strain

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C88A	is inactive	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
ubiquitin vinylsulfone	irreversible inhibitor that covalently modifies the active-site cysteines of DUBs	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	proteasome	Homo sapiens	-	-
additional information	proteasome	Bos taurus	-	-

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	Q9XSJ0	-	-
Homo sapiens	Q9Y5K5	-	-

Purification (Commentary)

Purification (Comment)	Organism
on Ni-NTA column	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
bone marrow	-	Homo sapiens	-
liver	-	Bos taurus	-
additional information	is highly abundant in the PA700 complex	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	neither Uch37 alone nor the Uch37-Adrm1 or Uch37-Adrm1-S1 complexes can hydrolyse di-ubiquitin efficiently, rather incorporation into the 19S complex is required to enable processing of polyubiquitin chains	Homo sapiens	?	-	?
ubiquitin-7-amido-4-methylcoumarin + H2O	-	Homo sapiens	ubiquitin + 7-amino-4-methylcoumarin	-	?

Synonyms

Synonyms	Comment	Organism
deubiquitinating enzyme	-	Homo sapiens
deubiquitinating enzyme	-	Bos taurus
DUB	-	Homo sapiens
DUB	-	Bos taurus
ubiquitin carboxy-terminal hydrolase	-	Homo sapiens
ubiquitin carboxy-terminal hydrolase	-	Bos taurus
UCH37	-	Homo sapiens
UCH37	-	Bos taurus