Literature summary for 3.4.19.12 extracted from

Das, C.; Hoang, Q.Q.; Kreinbring, C.A.; Luchansky, S.J.; Meray, R.K.; Ray, S.S.; Lansbury, P.T.; Ringe, D.; Petsko, G.A.
Structural basis for conformational plasticity of the Parkinson's disease-associated ubiquitin hydrolase UCH-L1 (2006), Proc. Natl. Acad. Sci. USA, 103, 4675-4680.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	enzyme activation modeling	Homo sapiens
additional information	enzymatic activation mediated by substrate binding	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of wild-type and mutant GST-tagged enzymes in Escherichia coli	Homo sapiens
subcloned from pcDNA-UCH-L1 vector into pGEX-6P-1 vector. The resulting N-terminally fused GST-tagged protein expressed in Escherichia coli Rosetta strain	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
at 2.4 A resolution, x-ray crystallography. Overall fold resembles that of other ubiquitin hydrolases, including UCHL3. Geometry of the catalytic residues in the active site of UCH-L1 is distorted in such a way that the hydrolytic activity appears to be impossible without substrate induced conformational rearrangements	Homo sapiens
purified recombinant wild-type and mutant GST-tagged enzymes, 35 mg/ml protein in 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 10 mM DTT, crystallization at room temperature, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular modeling, structure-function relationship	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C220S	sie-directed mutagenesis, structure analysis in comparison to the wild-type enzyme	Homo sapiens
S18Y	sie-directed mutagenesis, structure analysis in comparison to the wild-type enzyme	Homo sapiens
S18Y	monomeric in solution	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P15374	-	-

Purification (Commentary)

Purification (Comment)	Organism
by gel filtration	Homo sapiens
recombinant wild-type and mutant GST-tagged enzymes from Escherichia coli by glutathione affinity chromatography and gel filtration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	specific for	Homo sapiens	-
additional information	the enzyme is absent in tissues other than brain	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ubiquitin-7-amido-4-methylcoumarin + H2O	ubiquitin makes modest contact with UCH-L1. The interaction is mainly electrostatic, contributed by conserved acidic residues E7, E11, D30, E35, and E37	Homo sapiens	ubiquitin + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
dimer	crystallography	Homo sapiens
dimer	in crystal structure	Homo sapiens
monomer	in solution, sedimentation equilibrium analysis	Homo sapiens
monomer	in solution, sedimentation equilibrium experiments	Homo sapiens
More	structure-function relationship	Homo sapiens

Synonyms

Synonyms	Comment	Organism
PGP9.5	-	Homo sapiens
ubiquitin C-terminal hydrolase	-	Homo sapiens
ubiquitin hydrolase	-	Homo sapiens
UCH-L1	-	Homo sapiens

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Release 2023.1 (January 2023)