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Literature summary for 3.4.19.12 extracted from

  • Naito, S.; Mochizuki, H.; Yasuda, T.; Mizuno, Y.; Furusaka, M.; Ikeda, S.; Adachi, T.; Shimizu, H.M.; Suzuki, J.; Fujiwara, S.; Okada, T.; Nishikawa, K.; Aoki, S.; Wada, K.
    Characterization of multimetric variants of ubiquitin carboxyl-terminal hydrolase L1 in water by small-angle neutron scattering (2006), Biochem. Biophys. Res. Commun., 339, 717-725.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes Homo sapiens

Protein Variants

Protein Variants Comment Organism
I93M the mutation is linked to familial Parkinson's disease, the mutant dimer conformation is different from the wild-type enzyme deforming the globular form Homo sapiens
I93M wild-type enzyme, mutant I93M (linked to familial Parkinson's disease) and S18Y (linked to reduced risk of Parkinson's disease) are all self-assembled dimers. The configuration of these dimers are quite different. The wild-type is a rotating ellipsoidal Homo sapiens
S18Y the mutation is linked to reduced risk of Parkinson's disease, the mutant dimer conformation is different from the wild-type enzyme promoting the globularitiy Homo sapiens
S18Y wild-type enzyme, mutant I93M (linked to familial Parkinson's disease) and S18Y (linked to reduced risk of Parkinson's disease) are all self-assembled dimers. The configuration of these dimers are quite different. The wild-type is a rotating ellipsoidal Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28900
-
x * 28900, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens UCH-L1 is involved in Parkinson's disease ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal) UCH-L1 is a neuro-specific multifunctional enzyme, deubiquinating, ubiquitinyl ligase, and also stabilizing ubiquitin monomers, C90 is the active site residue Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
neuron
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UCH-L1 is involved in Parkinson's disease Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x '* 28900, SDS-PAGE Homo sapiens
dimer wild-type enzyme, mutant I93M (linked to familial Parkinson's disease) and S18Y (linked to reduced risk of Parkinson's disease) are all self-assembled dimers. The configuration of these dimers are quite different. The wild-type is a rotating ellipsoidal Homo sapiens
More the wild-type enzyme is a rotating ellipsoidal dimer, small-angle neutron scattering Homo sapiens

Synonyms

Synonyms Comment Organism
ubiquitin carboxyl-terminal hydrolase L1
-
Homo sapiens
UCH-L1
-
Homo sapiens