Cloned (Comment) | Organism |
---|---|
isozyme L1: expression of wild-type and mutants in Escherichia coli as His-tagged proteins | Mus musculus |
overexpression of isozyme L1 in mouse embryonic fibroblasts via adenovirus transfection | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C90S | site-directed mutagenesis, isozyme L1, inactive | Mus musculus |
C90S | exchange of the active site cysteine | Mus musculus |
D30K | site-directed mutagenesis, isozyme L1, inactive | Mus musculus |
additional information | gad mouse null mutants of isozyme L1 show reduced monoubiquitin level in neurons, overexpression of the isozyme L1 leads to an increased monoubiquitin level | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ubiquitin | isozyme L1 | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00016 | - |
ubiquitinyl-7-amido-4-methylcoumarin | 25°C | Mus musculus | |
0.00016 | - |
ubiquitin-7-amido-4-methylcoumarin | recombinant wild-type isozyme L1, pH 7.5, 25°C | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | isozyme L1 associates and colocalizes with monoubiquitin and elongates ubiquitin half-life, null mutants show a reduced monoubiquitin level in neurons, while overexpression causes an increase in monoubiquitin level, mechanism | ? | - |
? | |
additional information | Mus musculus | affects ubiquitin degradation and alters its metabolism. UCH-L1-mediated increases in ubiquitin levels are a function of UCH L1 affinity for ubiquitin rather than hydrolase activity. The enzyme insures ubiquitin stability within neurons | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
isozymes L1 and L3 | - |
Mus musculus | Q9R0P9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutants of isozyme L1 expressed in Escherichia coli | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | isozymes L1, specific for neuronal cells, testis and ovary, and L3 | Mus musculus | - |
additional information | no isozyme L1 expression in embryonic fibroblasts | Mus musculus | - |
additional information | isozyme L3 is universally expressed in all tissues | Mus musculus | - |
neuron | the enzyme insures ubiquitin stability within neurons | Mus musculus | - |
ovary | isozyme L1, specific for neuronal cells, testis and ovary | Mus musculus | - |
testis | isozyme L1, specific for neuronal cells, testis and ovary | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isozyme L1 associates and colocalizes with monoubiquitin and elongates ubiquitin half-life, null mutants show a reduced monoubiquitin level in neurons, while overexpression causes an increase in monoubiquitin level, mechanism | Mus musculus | ? | - |
? | |
additional information | affects ubiquitin degradation and alters its metabolism. UCH-L1-mediated increases in ubiquitin levels are a function of UCH L1 affinity for ubiquitin rather than hydrolase activity. The enzyme insures ubiquitin stability within neurons | Mus musculus | ? | - |
? | |
ubiquitin-7-amido-4-methylcoumarin + H2O | recombinant wild-type isozyme L1 | Mus musculus | ubiquitin + 7-amino-4-methylcoumarin | - |
? | |
ubiquitinyl-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | ubiquitin + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ubiquitin carboxy-terminal hydrolase L1 | - |
Mus musculus |
UCH | - |
Mus musculus |
UCHL1 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
ubiquitinyl-7-amido-4-methylcoumarin | 25°C | Mus musculus | |
0.02 | - |
ubiquitin-7-amido-4-methylcoumarin | recombinant wild-type isozyme L1, pH 7.5, 25°C | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
ubiquitin | 25°C | Mus musculus | |
0.0033 | - |
ubiquitin | pH 7.5, 25°C, recombinant wild-type isozyme L1 | Mus musculus |