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Literature summary for 3.4.19.12 extracted from

  • Osaka, H.; Wang, Y.L.; Takada, K.; Takizawa, S.; Setsuie, R.; Li, H.; Sato, Y.; Nishikawa, K.; Sun, Y.J.; Sakurai, M.; Harada, T.; Hara, Y.; Kimura, I.; Chiba, S.; Namikawa, K.; Kiyama, H.; Noda, M.; Aoki, S.; Wada, K.
    Ubiquitin carboxy-terminal hydrolase L1 binds to and stabilizes monoubiquitin in neuron (2003), Hum. Mol. Genet., 12, 1945-1958.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozyme L1: expression of wild-type and mutants in Escherichia coli as His-tagged proteins Mus musculus
overexpression of isozyme L1 in mouse embryonic fibroblasts via adenovirus transfection Mus musculus

Protein Variants

Protein Variants Comment Organism
C90S site-directed mutagenesis, isozyme L1, inactive Mus musculus
C90S exchange of the active site cysteine Mus musculus
D30K site-directed mutagenesis, isozyme L1, inactive Mus musculus
additional information gad mouse null mutants of isozyme L1 show reduced monoubiquitin level in neurons, overexpression of the isozyme L1 leads to an increased monoubiquitin level Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
ubiquitin isozyme L1 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00016
-
ubiquitinyl-7-amido-4-methylcoumarin 25°C Mus musculus
0.00016
-
ubiquitin-7-amido-4-methylcoumarin recombinant wild-type isozyme L1, pH 7.5, 25°C Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus isozyme L1 associates and colocalizes with monoubiquitin and elongates ubiquitin half-life, null mutants show a reduced monoubiquitin level in neurons, while overexpression causes an increase in monoubiquitin level, mechanism ?
-
?
additional information Mus musculus affects ubiquitin degradation and alters its metabolism. UCH-L1-mediated increases in ubiquitin levels are a function of UCH L1 affinity for ubiquitin rather than hydrolase activity. The enzyme insures ubiquitin stability within neurons ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
isozymes L1 and L3
-
Mus musculus Q9R0P9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutants of isozyme L1 expressed in Escherichia coli Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain isozymes L1, specific for neuronal cells, testis and ovary, and L3 Mus musculus
-
additional information no isozyme L1 expression in embryonic fibroblasts Mus musculus
-
additional information isozyme L3 is universally expressed in all tissues Mus musculus
-
neuron the enzyme insures ubiquitin stability within neurons Mus musculus
-
ovary isozyme L1, specific for neuronal cells, testis and ovary Mus musculus
-
testis isozyme L1, specific for neuronal cells, testis and ovary Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information isozyme L1 associates and colocalizes with monoubiquitin and elongates ubiquitin half-life, null mutants show a reduced monoubiquitin level in neurons, while overexpression causes an increase in monoubiquitin level, mechanism Mus musculus ?
-
?
additional information affects ubiquitin degradation and alters its metabolism. UCH-L1-mediated increases in ubiquitin levels are a function of UCH L1 affinity for ubiquitin rather than hydrolase activity. The enzyme insures ubiquitin stability within neurons Mus musculus ?
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O recombinant wild-type isozyme L1 Mus musculus ubiquitin + 7-amino-4-methylcoumarin
-
?
ubiquitinyl-7-amido-4-methylcoumarin + H2O
-
Mus musculus ubiquitin + 7-amino-4-methylcoumarin
-
?

Synonyms

Synonyms Comment Organism
ubiquitin carboxy-terminal hydrolase L1
-
Mus musculus
UCH
-
Mus musculus
UCHL1
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
ubiquitinyl-7-amido-4-methylcoumarin 25°C Mus musculus
0.02
-
ubiquitin-7-amido-4-methylcoumarin recombinant wild-type isozyme L1, pH 7.5, 25°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0033
-
ubiquitin 25°C Mus musculus
0.0033
-
ubiquitin pH 7.5, 25°C, recombinant wild-type isozyme L1 Mus musculus