Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.19.12 extracted from

  • Baek, S.H.; Woo, S.K.; Lee, J.I.; Yoo, Y.J.; Cho, C.M.; Kang, M.S.; Tanaka, K.; Chung, C.H.
    New de-ubiquitinating enzyme, ubiquitin C-terminal hydrolase 8, in chick skeletal muscle (1997), Biochem. J., 325, 325-330.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.19.12

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 59% inhibition at 5 mM Gallus gallus
KCl 92% inhibition at 0.2 M Gallus gallus
Mg2+ 61% inhibition at 5 mM Gallus gallus
Mn2+ 98% inhibition at 5 mM Gallus gallus
additional information no inhibition by PMSF, EDTA, and o-phenanthroline Gallus gallus
N-ethylmaleimide UCH-8 Gallus gallus
Ubiquitin aldehyde UCH-8 Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 2 * 130000, reducing SDS-PAGE Gallus gallus
240000
-
 UCH-8, gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
UCH-8, to homogeneity Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle pectoralis Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
branched polyubiquitin + H2O substrate is ligated to proteins via epsilon-NH-isopeptide bonds Gallus gallus ubiquitin
-
 ?
additional information no activity with poly-His-tagged di-ubiquitin Gallus gallus ?
-
 ?
ubiquitin-alpha-NH-dihydrofolate reductase + H2O
-
 Gallus gallus ubiquitin + dihydrofolate reductase
-
 ?
ubiquitin-alpha-NH-MHISPPEPESEEEEHYC + H2O
-
 Gallus gallus ubiquitin + MHISPPEPESEEEEHYC
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 130000, reducing SDS-PAGE Gallus gallus

Synonyms

Synonyms Comment Organism
ubiquitin C-terminal hydrolase 8
-
 Gallus gallus
UCH
-
 Gallus gallus
UCH-8
-
 Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 UCH-8 Gallus gallus
7.8
-
 assay at Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
7 9 little or no activity below pH 7.0 and above pH 9.0 Gallus gallus